A technique that can be used to separate proteins based primarily on the presence of non-polar residues on their surface is called A) ion-exchange chromatography. B) gel filtration chromatography. C) affinity chromatography. D) gel electrophoresis. E) hydrophobic interaction chromatography.

Answers

Answer 1

Answer:

E) hydrophobic interaction chromatography.

Explanation:

Hydrophobic interaction chromatography (HIC) is a methodology used to separate protein molecules depending on their hydrophobicity. HIC enables the separation and purification of proteins by using a reversible interaction between the target protein and the hydrophobic ligand of a HIC resin such as, for example, methacrylate-based beads. This interaction is influenced by the running buffer (high salt concentrations favor hydrophobic interactions). HIC enables the separation of proteins under low denaturation conditions, thereby preserving their biological functions.


Related Questions

A pseudogene is a Group of answer choices second copy of a gene that functions when the original copy becomes damaged. gene that is unrelated in sequence to another gene but has the same function. gene that evolved by gene duplication and the acquisition of mutations to yield a gene product that has a slightly different function from that of the original gene product. gene that arose through gene duplication, but by acquiring mutations became nonfunctional.

Answers

Answer: Gene that arose through gene duplication, but by acquiring mutations became nonfunctional.

Explanation:

A gene is a functional unit of heredity formed by DNA. They possess a sequence of nitrogenous bases that during translation is read by ribosomes to produce molecules called proteins.  A pseudogene is a DNA sequence that resembles a gene, but which has been inactivated in the course of evolution by mutations in its sequence. Thus, it is a gene that derives from other known genes and whose functions are different, may have lost their functionality or have radically changed it. To this day, it is not known exactly how pseudogenes are created, however some theories are as follows:

1. A gene duplication can generate two copies of a gene when only one is needed. A mutation would then occur that deactivates one of the copies. In addition, the duplication event may not be complete, so that the copy has incomplete promoters.  

2. They may be fragments of the messenger RNA transcript of a gene may be spontaneously reverse transcribed and inserted into the chromosomal DNA. They lack the promoters of normal genes, so they are not expressed normally.

3. A gene may become non-functional or inactivated if such a mutation becomes fixed in the population. This can occur by normal means such as natural selection or genetic drift.

Why was the scientific method developed?
Group of answer choices

to help scientists avoid religious disputes

to help scientists organize the process of solving problems

to confirm ancient facts about the Earth

to develop scientific laws that no one could argue with

Answers

Answer:

Its B

Explanation:

It just is

mark brainliest

what is the answer please help

Answers

Answer:

never become laws

Explanation:

trust

Isabel's first car had a manual stick shift. After many years of driving an automatic car, she needs to use her sister's car, which also has a stick
shift. Isabel takes a few minutes to get used to shifting gears. Which memory system Is she using to recall this information?
• A. episodic memory
O B. procedural memory
• C.
semantic memory
)D.
sensory memory

Answers

Answer:

Procedural memory

Explanation:

Isabel has to recall the information about using a manual stick shift. For this reason, she is using long-term, explicit, declarative and episodic memory system.

Human memory system can be broadly classified as: sensory memory, short-term memory and long-term memory.

Long-term memory is used to recall information. If the information are about facts, events and experiences then explicit, declarative and episodic memory helps to recall all information. This will help Isabel to recall all information about driving a car with a manual stick shift and she will be able to drive her sister's car.

(Answer taken from https://brainly.com/question/11839849)

What happens during S phase?

O A. Chromosomes are duplicated.

O B. DNA separates into two nuclei.

O C. The cell splits in two.

O D. Cytoplasm is manufactured.

Answers

Answer: A. Chromosomes are duplicated.

Explanation: This is the correct answer on the quiz.

Chromosomes are duplicated in S phase. Therefore, option (A) is correct.

What do you mean by S phase?

Between G1 phase and G2 phase, DNA replication takes place during S phase of the cell cycle. The processes that take place during S-phase are tightly controlled and highly conserved.

The cell replicates its genetic material entirely during the S phase of DNA synthesis; At the end of S phase, a normal diploid somatic cell with a DNA complement of 2N acquires a DNA complement of 4N.

DNA synthesis or replication occurs during the S phase of the cell cycle, which occurs prior to the interphase. Before entering mitosis or meiosis, the cell's genetic material is replicated in this manner, leaving enough DNA for daughter cells to divide.

Learn more about S phase:

https://brainly.com/question/10386886

#SPJ5

1. What academic subset of oceanography studies seafloor features and the processes that form them?
O A. Biological oceanography
O B. Marine geology
O C. Physical oceanography
O D. Chemical oceanography

Answers

Biology
Oxnh ma 7 acid

How fast does E. coli manifest symptoms A. 3 mins B. 3 hours C. 3 days D. 3 weeks

Answers

Answer:

C. 3 days

Explanation:

Answer:

c

Explanation:

2-5 days after exposure is when symptoms show

taken from the minnesota department of health

What is a likely consequence of continued human population growth? A. More rain forests B. Fewer urban centers Ο Ο Ο Ο O C. Less pollution D. Depleted natural resources​

Answers

Answer:

D. Depleted natural resources​

Explanation:

If the human population on Earth continues to grow, depletion of natural resources is a very likely consequence.

Especially at the rapid rate that humans consume natural resources, continued population growth would eventually deplete them.

Fossil fuels are an example of natural resources that will eventually deplete, as humans use immense amounts of things like coal and natural gas.

Answer choices A, B, and C are incorrect because they would be consequences of decreased human population growth.

So, the correct answer is D. Depleted natural resources.

suggest TWO reasons why fewer birds and other small animals can survive in the areas left after trees have been cut down​

Answers

Answer:

One is that they migrate to more fertile places.

Second, some animals may die because they can't stand the conditions of treelessness. But some animals can survive. because of adaptation

Explanation:

Removal of trees and other types of vegetation can reduce the availability of food, shelter and breeding habitat

What is Deforestation?

Deforestation also known as forest clearance is defined as the removal of forest or stands of trees from land that is converted to non-forest use in which forest land is converted into farms, ranches or urban may involve converting to use. The most concentrated deforestation occurs in tropical rainforests.

It occurs when humans remove or thin forests for timber or use land where trees stand for crops, grazing, extraction such as mining, oil, or gas, or as populations grow and people move. Development takes place in the form of migration.

Wildlife habitats are fragmented due to deforestation, where native species have to live on remaining habitat islands that are surrounded by disturbed land being used for agriculture and other uses.

Thus, removal of trees and other types of vegetation can reduce the availability of food, shelter and breeding habitat

Learn more about Deforestation, here:

https://brainly.com/question/11697527

#SPJ2

In which way could the results of an experiment have bias?
A)when samples sizes are large
B)an outlier data point is present
C)when sample sizes are chosen at random
D)data that has been evaluated for accuracy

Answers

Answer:

the answer is B if anybody is wondering

Explanation:

The way in which the results of an experiment have bias is an outlier data point is present. The correct option is B.

What is an experiment?

An experiment is a task performed in a hypothesis to prove the results of the experiment.

The experiment needed the required instrument, chemicals, products, etc.

Thus, the correct option is B) an outlier data point is present.

Learn more about experiment

https://brainly.com/question/11256472

#SPJ2

Cells in the human body carry out different functions. Which cells help the body fight off diseases and remain healthy? Choose the two that apply.

Answers

Phagocytes and lymphocytes

Which level of organization is characterized by a group of cells that work together to perform a common function?
organ
tissue
organ system
organism

Answers

Answer:

B

Explanation:

Answer:B

Explanation:

2. Sam and Jerry are having a discussion in their Environmental Science lass. Sam
says that abiotic factors determine what biotic factors can be in an ecosystem. Jerry
says that biotic factors determine what abiotic factors can be in an ecosystem. Who
is correct? Use an example to support your answer. (2 points)

Answers

Answer:

Jerry is right.

Explanation:

Abiotic factors can kill biotic factors, so technically Jerry is correct, just think of it as a TDI game, let's say Chris is an Abiotic factor, and the campers are biotic factors, Chris chooses weather or not they stay.

Heather is doing an acid-base reaction. She has 25.00 mL of hydrochloric acid of unknown concentration in a flask. She wants to add a basic solution to the acid drop by drop, until all the acid has reacted.

Answers

Answer:

lwlwlelpepwpspsppa

Explanation:

ookeieorooroekrkrkrklrkdkdodkekiekeieie

This type of behavior is important to...?

Answers

Maintain homeostasis. Small lizards are cold blooded so they need to sunbathe in the morning to maintain their body temperature so that they can obtain nutrients from digestion.

25 puntos ayudame biologia gracias

Answers

Answer:

no puedo entender tu idioma

reaccionar unos con otros navegar por Internet y buscarlo

Scientists at the tar pits discovered that many of the smaller animals they extracted from the pits still exist around Los Angeles today. But many of the larger mammals-such as woolly mammoths and saber-toothed tigers-are now extinct. Why do you think this is the case? What can scientists learn about Los Angeles' climate history by studying the specimens found in these tar pits?

Answers

More than one million bones were recovered in 1906. These bones represented 231 species of vertebrates and there were 234 species of invertebrates.

Los Angeles climate is relatively stable for over 50,000 years. The fossils of more than 180 insects were preserved in La Brea Tar Pits. The climate warming probes a link for evolution of Ice Age predators.

Learn more at https://brainly.com/question/14442796

Answer:

scientists at the tar pits discovered that many of the smaller animals they extracted from the pis still exist around los angeles today. But many of the larger mammals such as wooly mammoths and saber toothed tigers are now extinct. I believe this is the case because since bigger beasts couldnt take shelter in small protected burrows, perhaps they were done in by fierce environmental conditons. Or maybe with so many plants dying off, big herbivores simply had nothing to eat, and as they died out, so did the big carnivores.La brea tar pits are probing the link between climate warming and the evolution of ice age predators, attempting to predict how animals will respond to climate change today.

Explanation:

Which of the following refers to the movement of biochemical and other atomic or molecular substances across biological membranes?
Membrane transport
ATP breakdown
Diffusion
Osmosis

Answers

Answer:

diffusion / osmosis passive transport does not require an input of chemical energy.

Explanation:

Which of the following statements are true regarding science?
All of the above are correct

Scientists collect and organize information in a careful, orderly way, looking for patterns and
connections between events.

Scientists propose explanations that can be tested by examing evidence.

Science deals with the natural world.

Answers

Answer:

All of the above are correct

The chemical forces that contribute to the stability of the DNA helix are (choose the best answer):______

a) hydrogen bonds van der

b) Waals interactions

c) disulfide bonds

Answers

a) hydrogen bonds van see

The forces that contribute to the stability of DNA helix are hydrogen bonds. That is option A.

Deoxyribonucleic acid (DNA) is a molecule found within nucleus of cells which contains genetic information that is used by the organism for development.

A molecule of DNA is made up of four nitrogenous bases which include:

Adenine (A), Cytosine (C), Guanine (G), andThymine (T)

The DNA helix is double which is made up of two linear strands that run opposite to each other and twist.

Hydrogen bond is defined as the intermolecular force that is capable of forming a dipole-dipole attractions.

The hydrogen bond that is formed between the nitrogenous base pair helps contributes to the stability of the DNA helix.

Therefore, the forces that contribute to the stability of DNA helix are hydrogen bonds.

Learn more here:

https://brainly.com/question/21992450

In a rainforest, there are trees of different sizes as shown in the figure below.
The trees in the UNDERGROWTH layer will probably face competition for which of the following resources?

a. Air
b. Water
c. Animals
d. Sunlight

Answers

Animals is the correct answer hope this helps

Last electron level that has an electron in it.
Group of answer choices

Valence Level (Shell or Cloud)

Atom

Electrons

Atomic Symbol

Answers

Valence level (shell or cloud)

Why are NADH and FADH2 necessities in the electron transport chain?(1 point)

They power the electron transport chain.
They power the electron transport chain.

They remove electrons from the electron transport chain.
They remove electrons from the electron transport chain.

They move down the electron transport chain.
They move down the electron transport chain.

They move electrons to the electron transport chain.

Answers

NADH and FADH2 are necessities in the electron transport chain because they power the chain by moving electrons into it.

NADH and FADH2 are both generated at the initial stages of respiration (the Kreb's cycle), a process during which food materials are broken down to generate energy. NADH is generated from NAD+ as a result of the addition of electrons while FADH2 is generated from FAD also due to the addition of electrons.

Once generated, both compounds get transferred into the innermost membrane of the mitochondrion where they travel down the electron transport chain, donating their electrons in the process and creating an electrochemical gradient to generate ATP.

Without NADH and FADH2, there will not be electrons to power the electron transport chain and the generation of ATP would become impossible.

More about the electron transport chain can be found here: https://brainly.com/question/13975046

Answer:

The correct answer is They move electrons to the electron transport chain.

Explanation:

just took the quick check!

hope this helps :)

calculate the distance covered by a school bus in 2 minutes moving with a velocity of 30m/s

explain also..​

Answers

Speed=30m/sTime=2min=120s

We know

[tex]\boxed{\sf Distance=Speed\times Time}[/tex]

[tex]\\ \sf\longmapsto Distance=30\times 120[/tex]

[tex]\\ \sf\longmapsto Distance=3600m=3.6km[/tex]

latex from _______ is the source of biodiesel
A. Jatropa
B. Tulasi
C. Neem
D. Cactus​

Answers

Latex from cactus is the source of biodiesel.

Identify the TRUE statement about fossil preservation: Group of answer choices A dead organism will most likely become a fossil if there IS abundant oxygen in the depositional environment. Low-energy environments are NOT suitable for preserving fossils. Organisms with hard parts made of shell or bone are more likely to become fossilized. The slower a dead organism is buried by sediment, the more likely it is to become a fossil.

Answers

Answer:

Organisms with hard parts made of shell or bone are more likely to be fossillized.

Explanation:

Bone/ cartilage/ shell decompose slower than other parts of organizims. If an organism is in regular fossilization conditions, then it is most likely to become a fossil.

What is one way private citizens can help conserve natural resources?



Answers

Answer:

By reducing their use of energy, water, and waste services

Please help me...will give braniliest

Answers

The answer is segment Segment
Segmented


Hope it helps…Good luck..Stay safe and healthy…thanks….<3333

which structures in plant cells build protein molecules under the control of the nucleus?
a) cell walls
b) chloroplasts
c)mitochondria
d) ribosomes

Answers

The cell walls, chloroplasts, and mitochondria play important roles in plant cells, they are not directly involved in building protein molecules under the control of the nucleus. D

Ribosomes are the structures in plant cells that build protein molecules under the control of the nucleus.

Ribosomes are tiny, complex molecular machines composed of RNA and protein molecules.

They are involved in the process of protein synthesis, where they facilitate the translation of genetic information from the DNA in the nucleus into functional proteins.

The nucleus of a cell contains the DNA, which serves as the blueprint for building proteins.

However, the actual synthesis of proteins occurs in the cytoplasm, outside the nucleus.

Ribosomes are responsible for this task.

Ribosomes can be found both freely floating in the cytoplasm and attached to the endoplasmic reticulum (ER).

The ones attached to the ER are called rough endoplasmic reticulum (rough ER) due to the presence of ribosomes on their surface.

These ribosomes associated with the rough ER are involved in the synthesis of proteins that are destined for secretion or for integration into the cell membrane.

The process of protein synthesis involves the decoding of the genetic information stored in messenger RNA (mRNA) by ribosomes.

Ribosomes read the mRNA sequence and assemble amino acids into a growing polypeptide chain, forming a functional protein.

Cell walls provide structural support, chloroplasts are responsible for photosynthesis, and mitochondria are involved in cellular respiration. However, the primary site for protein synthesis in plant cells is the ribosomes.

Therefore, the ribosomes (option d) are the structures in plant cells that build protein molecules under the control of the nucleus.

For similar questions on plant cells

https://brainly.com/question/29263392

#SPJ8

Nxnznmznznnznznnzndnxnznnxnznxnznznznz

Answers

Hehehdjdjjciifeisodod
Other Questions
State and explain the trend in electronaffinity shown by halogens If n(a)=40,n(b)=60 and n(ab)=80.find the value of (ab. In a birthday party, a cake was cut into 30 equal sizes pieces. At the end of the party, 8 pieces were left. What was the percentage of cake consumped? what is the area of triengle if b=6cm and h=4 cm. please explain it and do all the process Explain 2 problems Hunters and Gatherers would have faced in their daily lives? Explain your answer Which of the following is the best definition of a physical property?A. Tearing paper in half.B. Something that can be observed or measured while changing the identity of the substance.C. The ability of something to undergo a change or reaction.D. Something that can be observed or measured without changing the identity of the substance. Click on the graphic to select the figure that would make the following "a reflection in line k." of Defers the to Breh medias . 2 Vastel 3. Fosil 5. 4- Adult frardise Marifesto 1.2 Very Very heat According to the definitions used by the USDA Economic Resource Service, what percentage of U.S. households were, at times, uncertain about having enough food to meet needs, but generally obtained enough food to avoid substantially disrupting their eating patterns by using a variety of coping strategies Look at this cubeIf the side lengths are halved, then which of the following statements about its surface area will be true? solve for the value of C Sam is shopping for clothes. There's a 9% sales tax where he lives. Select the expression that gives the price of clothes after including the sales tax, where c is the original cost of the clothes.1c + 0.9cIncorrect Response1.09c + 0.09cCorrect Answer1.09c Please help ASAP!!!! factorise completely 4x^(2 )(x + 1) - 6x (x+1) What is the correct answer?IT IS NOT PREPOSITION!! 7points can someone help? Please Need someone to check this for me Use FORMAL USTED COMMANDS to give directions from where you live to the place you indicated in #2. Add specific information, like street names and landmarks, so that the person could get there without getting lost. Include formal commands for at least three different verbs. ANSWER: Siga por Phoenix Road hasta llegar a una interseccin. Doble a la derecha. Bajars una colina. siga recto hasta llegar a la carretera de Meyer. Gire a la izquierda. Parar en la seal de parada. Espere a los peatones. Ahora gire a la izquierda hacia el estacionamiento. Llegaste!Question number 5: Write 4 sentences about yesterday. Start out by telling what the weather was like, and then write 3 more sentences describing your activities at different times of the day.ANSWER: Ayer el tiempo estuvo clido todo el da. A las ocho de la maana corr y com en un caf. A las doce de la tarde trabajaba. A las seis de la noche cen con mi familia. use words with appropriate connotationplease help me Hot-dog buns come in packages of 10. Wieners come inpackages of 12. Barry would like to buy the smallestnumber of hot-dog buns and wieners so that he will haveexactly 1 wiener per bun. How many packages of hot-dogbuns and wieners must he buy?A 6 packages of buns, 5 packages of wienersB 5 packages of buns, 5 packages of wienersC 8 packages of buns, 7 packages of wieners05 packages of buns, 4 packages of wieners Modern periodic table is based upon atomic number. Explain what does the modern periodic table states about the atomic numbers ?