A research group discovers that a reagent can be used to form covalent cross-links between various types of atoms in both small molecules and proteins. They add the reagent to a mixture of 14C-lactose and a protein that is thought to have a specific binding site for lactose. What should they do next to draw a conclusion about whether the protein specifically binds lactose

Answers

Answer 1

Answer and Explanation:

Ideally, researchers would measure the stoichiometry of the mixture labeled with 14C-lactose and a protein. By measuring this stoichiometry, researchers should be able to find a concentration between lactose and protein of 1:1. This concentration is able to determine that the protein has specifically bound to lactose.

In summary, to determine whether the protein binds lactose, researchers need to calculate whether the concentration of these two elements within the mixture is 1:1.


Related Questions

How do bacteria obtain energy to carry out their functions

Answers

Answer:

Bacteria can obtain energy and nutrients by performing photosynthesis, decomposing dead organisms and wastes, or breaking down chemical compounds. Bacteria can obtain energy and nutrients by establishing close relationships with other organisms, including mutualistic and parasitic relationships.

Explanation:

Where would you expect to find a mountain range caused by the collision of two continental plates on the map below? (100 points)

Public Domain


A

B

C

D

Answers

The correct answer is A.

Explanation

The image shows a map with the tectonic plates of the earth and four points are marked with the letters from A to D. The letter A indicates the collision of the Eurasian Plate and the Indian plate. The letter B indicates the border zone between Russia and the United States, however, there is no plate collision. The letter C marks are on the coast of Antarctica, but there is no collision between two plates. The letter D indicates an area on the equator, but it is not located on any plate collision. Therefore, the correct answer is A that shows the collision of the Eurasian Plate and the Indian plate where the great mountain chain of the Himalayas is formed where Mount Everest is located, the highest in the world with its summit at 8,849 meters above sea level. This is because many of the great mountain ranges of the world originate from the collision of plates such as the Andes and the Himalayas.

Please help;
Given the range of current speeds shown on the graph above, would deposition ever be possible for clay (0.001 mm) or silt (0.01mm) particles?
Answer yes or no and explain your answer.

Answers

Answer:

Yes.

Explanation:

Yes, deposition can be possible for clay (0.001 mm) and silt (0.01mm) particles when the agent that carry its load is absent in that region such as wind. Wind is the agent that moves clay and silt particles with it so if there is high wind in a specific region, no deposition will occur while on the other hand, those areas where wind is not present, deposition will definitely occurs so we can say that deposition depends on the presence and absence of wind.

What two aspects of internal control must the auditor assess when performing procedures to obtain an understanding of internal control?

Answers

The auditor must obtain an understanding of and perform tests of controls related to all significant account balances, classes of transactions, and disclosures and related assertions in the financial statements.

Name one geographic region that has few or no fossil primates and explain why.

Answers

Answer:

Australia

Explanation:

Fossils are preserved remains (or mouldings) of ancient living things which are preserved in sedimentary rocks. Primate fossils are generally found along with other fossils, usually in ancient soils that contain information on the conditions under which they were formed. Primate fossils are generally found in tropical and subtropical regions (which represent the natural habitat in which these species live). Primates never lived in Australia, and therefore no fossil primates from this geographic area have been found.

Do you think that organisms of the same order will share a stronger evolutionary relationship than organisms that share only the same phylum? Explain your reasoning using the results of your investigation.

Answers

Answer:

In taxonomic, the organism is classified based on some similarities. ... But the organism with the same order should have the same phylum and class too since order is located below the phylum. That means the organism with the same order should have more similarities than the organism with the same phylum.

Taxonomical classifications are done for organisms including plants, animals, microorganisms etc. It classifies, defines and scientifically names organisms.

According to the taxonomical classification the organisms are classified based on similarities and in order of the taxonomy, the organism should have the same phylum and class.

In taxonomy, the phylum is above the order in level and the phylum will have fewer similarities compared to the organism in order or class.

The organism of the same order will have a strong evolutionary relationship as compared to the organism with the same phylum.

To learn more about taxonomical classification follow the link:

https://brainly.com/question/1918761

In a study involving a cell-free translation system from coli, the polyribonucleotide AUGUUUUUUUUUUUU directs the synthesis of the oligopeptide fMet-Phe-Phe-Phe-Phe. In the presence of a new class of translation-targeting antibiotics that bind to the large ribosomal subunit, the ribosomal complex forms, the initiator tRNA binds the mRNA, the second tRNA enters the A site of the ribosome, but no peptide bonds form. Which of the following is a likely function of these new antibiotics?

a. Blocks peptidyl transferase activity
b. Inhibits tRNA aminoacyl synthetase function
c. Inhibits ribosomal translocation along the mRNA
d. Prevents anticodon base-pairing with the codon
e. Blocks binding of the Shine-Dalgarno box to the rRNA

Answers

Answer:

c. Inhibits ribosomal translocation along the mRNA.

Explanation:

Initiator tRNA is thought to bind directly to P-site of small ribosomal sub unit. These tRNA are positioned at P site and remain attached to tRNA located at this site. Initiation factor helps to mediate this event.

Imprinting can be cell-type specific; for example, this region of chromosome 15 is ONLY imprinted in neurons and a few other specific cell types, but it is not imprinted in most cells in the body, such as bone, liver, and heart cells. Which of the following statements are true about a healthy individual without Prader-Willi or Angelman syndrome? ( Choose two answers)
a. Maternal and paternal copies of SNRPN are expressed at the same level in heart cells.
b. The maternal and paternal copies of UBE3A are differentially expressed in bone cells.
c. The maternal copy of SNRPN has DNA methylation at its promoter in liver cells.
d. Expression of the paternal copy of UBE3A is silenced in neurons.

Answers

Answer:

The correct answer is - A and D.

Explanation:

Genetic imprinting is DNA modifications that result in changing the expression but not the sequence. Factors That affect such changes present both within and outside the cell.

It is a result of the silencing of either maternal or paternal alleles in a pair. This occurs random and leads to phenomenon like X-inactivation. The mechanism involves methylation of the DNA to be silenced. paternal copy of UBE3A is silenced in neurons.

2.
How do scientists classify intrusive igneous features?
according to shape, texture, and their distance from the nearest volcanic field
according to shape, color, and their relationship to surrounding layers of rock
according to shape, size, and their relationship to surrounding layers of rock

Answers

Answer:

according to shape, size, and their relationship to surrounding layers of rock

Explanation:

Intrusive igneous features are those formations around the igneous rock that are developed over time when magma cools and forms a solid shape, and they may include stocks, sills, di.kes, etc.

Therefore, scientists classify intrusive igneous features according to shape, size, and their relationship to surrounding layers of rock.

which statement describes a process associated with meiosis

Answers

Answer:

homologous chromosomes pair up

Answer:

Haploid cells join to form an organism that has a complete set of chromosomes.

Explanation:

In New York, the worst-hit state, a total of 350,121 cases have been confirmed and 28,232 deaths have been reported. There are now 29 states with tens of thousands of cases, and more than 50,000 in New York, New Jersey, Massachusetts, Illinois, California, Pennsylvania and Michigan

Answers

Answer:

Thats true

Explanation:

Describe fluid exchange between capillaries and the interstitial fluid. Be sure to discuss the forces (pressure) that moves the fluid and the direction in which fluid moves. (4 points) In one to three sentences, describe the role of lymphatic system in this fluid exchange. (3 points) g

Answers

Answer:

Capillary exchange is the exchange of fluid from the capillaries into the interstitial fluid and vice versa. Diffusion, transcytosis, and bulk flow are three mechanisms that facilitate capillary exchange.

The blood plasma or interstitial fluid exerts a pressure of the fluid on the capillary walls known as Hydrostatic pressure. Osmotic pressure is a pressure exerted by proteins either in the blood plasma or interstitial fluid called oncotic pressure.

Lymphatic capillaries gather lymphatic fluid and regulate the pressure of interstitial fluid due to the forces of hydrostatic or oncotic pressure. An essential role of the lymphatic system is to return the fluid to the blood.

Which of the following are the three areas of the tundra biome? (Choose all that apply.)
Arctic
Asian
Antarctic
alpine

Answers

Answer:

The three areas of the tundra biome are artic, antarctic and alpine.

Which level of organization includes all the other levels of organization?

Answers

answer : biosphere

from brainly

LEGEND778

Genius

5.5K answers

7.3M people helped

Answer:

The highest level of organization for living things is the biosphere; it encompasses all other levels. The biological levels of organization of living things arranged from the simplest to most complex are: organelle, cells, tissues, organs, organ systems, organisms, populations, communities, ecosystem, and biosphere.

Answer:

biosphere

The highest level of organization for living things is the biosphere; it encompasses all other levels. The biological levels of organization of living things arranged from the simplest to most complex are: organelle, cells, tissues, organs, organ systems, organisms, populations, communities, ecosystem, and biosphere.

Hope this helps!

Please give me brainliest if this helped you!

what is osmolarity of mammalian urine?​

Answers

Answer:

The osmolarity of mammalian urine may vary over time. The osmolarity of mammalian urine varies little between species. Mammalian urine is always hyperosmotic to blood. The osmolarity of mammalian urine may vary over time.

Explanation:

I hope it will help you

Type of symbiosis Suppose friendly ants fed on sugary droplets excreted from a caterpillar, but did nothing to help the caterpillar. Assuming that taking the sugar does not hurt the caterpillar, what type of symbiotic relationship is this

Answers

Answer:

Commensalism

Explanation:

All organisms in nature interact with other organisms in different ways. Symbiotic interactions involve a relationship between two or more organisms of different species in which one or all of them turn to be beneficiated by that relationship. These species create permanent mutual dependence.  

Symbiotic interactions include:  

• Amensalism refers to a biological relationship established between two organisms, in which one of them avoids the growth, development, or survival of the other one. In this interaction, one of the organisms seems to be strongly affected by the other one. Meanwhile, the other organism does not seem to be affected at all.  

Commensalism: In this case, the fitness of only one of the species seems to increase, while the other species is not affected at all, nor positively or negatively. The unaffected species might provide food or refuge to the other species, which is benefited.  

• Parasitism: In this case, one of the species is beneficiated, while the other suffers a consequence, reducing its fitness. These interactions are mainly about nutrition, although there might be other benefits too. Some parasite species might coexist with their host, while some others might get them ill to the point of causing death.  

• Mutualism: The interaction is favorable for both species. Their fitness increases with these interactions, although they do not need this relationship to survive. The degree of benefits might vary among species or might be equal.

-----------------------------------------------------------------------------------------------------------

The exposed symbiotic example is a commensalism case. The ant is benefited from taking the sugary droplets. Its fitness increases. But ants do not make any harm to the caterpillar. In fact, it has no effect on them at all, no possitive nor negative. The caterpillar´s fitness is not influenced by the ant.

Describe what is happening in each of the phases of cellular division with what you observe from the photomicrographs in the lab instructions (describe and compare both).

Answers

Answer:

Interphase - There are 3 subphases called - G1 , S and G2 . In these subphases, DNA material is duplicated, and essential proteins for cell division are formed.

Prophase - the nuclear envelop and nucleolus disappears and condensed DNA forms chromosomes that are short in size.

Metaphase - the form of two sister chromatids are arranged in the middle of the metaphase/equatorial line. Two centrioles are found at two poles of the cell which initiate the formation of spindle fibers.

Anaphase - spindle fibers are formed and can be observed. These fibres attach with the kinetochore present in the centromere of chromosomes and these chromatids now start separated by pulling and pushing force of spindle fibers in opposite poles

Telophase -  chromatids reach at destination pole and then nuclear envelop starts creating again in both poles. Nucleolus reappears and now in a cell two nucleus are found.

b) The chemical equation below summarizes photosynthesis.
Energy (sunlight) + 6 H20 + 6 CO2 - C6H1206 + 6 02
Write a similar chemical equation for cellular respiration. Be sure to include a
description of the form of energy that results from cellular respiration. (3 points)
Pls help me

Answers

Answer:

The chemical equation for cellular respiration would be: C6H12O6 + 6 O2 → 6 CO2 + 6 H2O.

Explanation:

C6H12O6 (1 Glucose molecule) + 6 O2 (6 Oxygen molecules)  → 6 CO2 (6 Carbon dioxide molecules) + 6 H2O (6 Water molecules)

DNA and RNA are structurally similar in some ways but different in others. Identify whether each of the statements applies to DNA, RNA, both RNA and DNA, or neither DNA nor RNA.

Answers

What are the statements?

geosphere burying organisms
hydrosphere respiration of marine life
biosphere photosynthesis atmosphere burning things

Answers

Answer:

This question is incomplete, the question is asking to match each activity with the sphere to which it adds carbon or CO2.

ANSWERS:

Geosphere = burying organisms

hydrosphere = respiration of marine life

biosphere = photosynthesis atmosphere = burning things

Explanation:

This question describes the types of spheres we have. The respective activities that occur in each sphere that adds CO2 or carbon are as follows:

- Geosphere: This refers to the solid part of the Earth. Dead organisms are buried in the geosphere (soil) where their carbon remains can be deposited.

- Hydrosphere: This constitutes all of the water bodies (sea, river, stream, ocean) on the Earth. When marine organisms respire in water, carbon dioxide (CO2) is released.

- Biosphere: A sphere consisting of all living things (plant, animal, microbes etc.) on Earth. Plants as a living organisms photosynthesize and make use of carbon dioxide to produce glucose, which stores in the plant.

Atmosphere: The gaseous component of the Earth. Burning substances release carbon based gases into the atmosphere.

What would be most likely to happen if some of the proteins Ik one of the photo systems were to change shape due to a drop in cell pH?
A. A decrease in the formation of NADPH
B. An increase in the formation of NADP+
C. A decrease in chlorophyll production
D. An increase in sugar production

Answers

Answer:

Option A

Explanation:

If some proteins in one of the photosystems undergo change in their shape due to a drop in pH of cell, they will not perform their activity. Due to this, there will be a decrease in the formation of NADPH ( that is less reduction of NADP due to blockage in the transport of electron).

A new member of your team is visually impaired. He's having a hard time reading his computer screen. How can you show that you value diversity? a) Suggest the organization may not be a great fit. O b) Tell him to apply for a different job within the organization that does not b) require computer usage. 12 Od Tell him that he might want to schedule an eye exam. c) d) Provide access to technology that can magnify or read what is on the computer screen.

Answers

Explanation:

Provide access to technology that can magnify or read what is on the computer screen.

what is the effect of increasing the concentration of each of the following metabolites on the net rate of glycolysis; 1.glucose 6-phosphate. 2. Fructose 1,6-bisphosphate 3. citrate 4. Fructose 2,6-bisphosphate​

Answers

Answer:

hlo everyone how are you all ☆☆☆

How does temperature and concentration of monounsaturated phospholipids change the rate at which molecules permeate the plasma membrane? Does this change occur equally for low permeability ions the way and large molecules as it does for gasses and high permeability molecules?

Describe the way that enzymes increase the rate of chemical reactions in the cell. What other factors related to enzymes can increase or decrease this rate and why?

Answers

Answer:

я не знаю ответа извините

Explanation:

Why is the frequency of natural disasters increasing?

1. There are less natural hazards occurring

2. Human population in areas prone to natural hazards has increased

3 . Deaths from natural disasters has decreased in developed countries and increased in developing countries.

Answers

Human population in areas prone to natural hazards has increased

Why is the frequency of natural disasters increasing?

1. There are less natural hazards occurrin

2. Human population in areas prone to natural hazards has increased

Explain how a bottleneck event can enrich recessive traits in the genetics of a population.

Answers

Answer

A bottleneck event severely reduces the fraction of a species able to pass on their genes to offspring. Any once rare recessive traits present in the surviving breeding population are passed to a larger fraction of the total population of offspring, thereby enriching those recessive traits in the total gene pool .

Bottleneck events can limit variation if the dominant allele population gets wiped out for any reason and the recessive allele can appear to be in the majority however this does not shows the actual makeup of the population.

What is bottle neck effect?

The bottleneck effect is defined as a reduction and subsequent increase in a population's size affects the distribution of genetic variation among its individuals.

Examples of bottle neck effect  is when the northern elephant seals have reduced genetic variation likely because of a population bottleneck humans imposed on them in the 1890s. Hunting reduced their population size to as few as 20 individuals at the end of the 19th century.

For more information regarding bottleneck effect, visit:

https://brainly.com/question/8898854

#SPJ2

Which two events are part of the rock cycle?
O A. Carbon cycles between plants and animals.
B. Plants take minerals from soil and grow.
C. Melted rock cools and solidifies.
D. Sediment is buried and compacted.

Answers

C: melted rock cools and solidifies

D: sediment is buried and compacted

Answer:

1) Sediment is buried and compacted

2) Melted rock cools and solidifies

Explanation:

Trust me love

is important to the transmission of nerve impulses.
a) Zinc
b) Calcium
c) Sodium
d) Iron
Please help thx

Answers

is important to the transmission of nerve impulses.

a) Zinc

b) Calcium

c) Sodium

d) Iron

[tex] \bold \pink{sodium}[/tex]

Answer:

B)Calcium

Explanation:

Have a nice day

Mention the four characteristics of warm blooded animals.​

Answers

Answer:

They maintain their body temperature higher than their environment by some metabolic processes like contraction of muscles, friction produced by flow of blood etc. They have a higher basal metabolic rate, and also a greater capacity to increase their metabolic rate.

In the test tube channel below what is produced by the snail that is used by the plant?

Answers

Where is the channel below?
Other Questions
factorise 1000a^2+27b^2 Rough-cut capacity planning: Multiple Choice Looks at specific products to be run in specific factories. Determines if the MRP is feasible or not. Analyzes both labor and equipment capacity throughout the organization. Examines total capacity by measuring average factory output. He said, "I've been playing football for three hours." (Into indirect speech) John downloaded the manual for his TV, called manual-of-tv.pdf, from the manufacturer's website. After he clicked twice on the document he was informed by Windows that the file could not be opened. Which software must John install to solve the problem? A Geiger counter registers a count rate of 8,000 counts per minute from a sample of a radioisotope. The count rate 24 minutes later is 1,000 counts per minute. What is the half-life of the radioisotope? You and your best friend are both on the swim team. You want to beat your friend at the next swim meet so you decide to swim 15 minutes longer than she does one day at practice. Write an equation for the number of minutes you swim, y, when your friend swims x number of minutes. You swim 55 minutes. How many minutes did your friend swim? (1 point) There were 23.7 million licensed drivers in California in 2009 and 22.76 million in 2004. Find a formula for the number, N, of licensed drivers in the US as a function of t, the number of years since 2004, assuming growth is (a) Linear N(t) write the process of the formation of district co ordination committee Information technology (IT) consists of all the hardware that a firm needs to use in order to achieve its business objectives, whereas information systems consist of only the software and business processes.A. TrueB. False Economists assume that individual decisions will be determined by the output or production costs they create. the output or production costs they create. the trade-offs they creates. the trade-offs they creates. the societal demands associated with them. please explain this to me? NEED ASAP!!!In this figure, AB || CD and m/_3=120 What is m/_6? Enter your answer in the box Analysis of Apple's innovation activities is the process of modifying something to make it fit certain criteria. Jeff has one month to prepare for his debate. What should he do to best prepare his argument? Check all that apply.O consider the various perspectives of each sideresearch the topic from others' points of viewOpick out an appropriate outfit for the debatecompare opposing viewpoints to his owncraft thoughtful and respectful rebuttals The "Pressure" meter allows you to read the pressure at different depths in the fluid. Place the pressure meter close to the bottom of the pool, and read the pressure. Slowly move the pressure meter toward the surface of the water in the pool and read the pressure at different depths in the pool. What happens to pressure in the fluid as the depth of the fluid decreases? Determine the product of (46.2 10^-1) (5.7 10^6). Write your answer in scientific notation.A) 2633.4 10^5B) 2.6334 10^7C) 2.6334 10^1D) 2.6334 10^5 Answer the question Why is page one of the story the importance of being earnest important?Scene: Morning-room in ALGERNON'S flat in Half Moon StreetThe room is luxuriously and artistically furnished. Thesound of a piano is heard in the adjoining room. LANE isarranging afternoon tea on the table, and after the music hasceased, ALGERNON enters.ALGERNON. Did you hear what I was playing, Lane?LANE. I didn't think it polite to listen, sir.ALGERNON. I'm sorry for that, for your sake. I don'tplay accurately any one can play accurately but Iplay with a wonderful expression. As far as the piano isconcerned, sentiment is my forte. I keep science forLife.LANE. Yes, sir.ALGERNON. And, speaking of the science of Life, haveyou got the cucumber sandwiches cut for Lady Bracknell?LANE. Yes, sir. (Hands them on a salver.)ALGERNON. (Inspects them, takes two, and sits down on thesofa.) Oh! ... by the way, Lane, I see from your book thaton Thursday night when Lord Shoreman and Mr. Worthing were dining with me, eight bottles of champagne are entered as having been consumed.LANE.Yes, sir; eight bottles and a pint.ALGERNON.Why is it that at a bachelors establishment the servants invariably drink the champagne? I ask merely for information. Why does Jefferson think the National Bank is unconstitutional