Read the instructions for this self-checked activity. Type in your response to each question, and check your answers. At the end of the activity, write a brief evaluation of your work. Activity Plasmid, a DGFPuy developed by Clontech, contains a gene for the green fluorescent protein (GFP) that comes from the bioluminescent jellyfish Aequora victoria. This gene allows the jellyfish to produce a protein that fluoresces, or glows, under ultraviolet light. GFPuv is used as a vector to transform E.coli bacteria. The GFP gene of GFPuv is switched on in the presence of lactose. The plasmid also contains a gene for ampicillin (antibiotic) resistance. Cells with Amor produce a protein that decomposes ampicillin and enables them to survive in an ampicillin environment. Perform the following steps in a laboratory:
1. Introduce the plasmid PGFPUV in E.coli cells by subjecting them to heat shock in the presence of calcium chloride.
2. Spread samples of these cells on four sterile agar plates. Three plates should contain nutrient agar (NA) and one should contain Luria Bertani agar (LB) NOTE LB agar contains lactose. NA contains glucose. Based on the treatment meted out in each plate, write down your observations and conclusions. Compare the results with your peers.
Treatment. observation
-DNA NA.
-DNA/NA / Amp
+DNA NA / Amp
+DNA/LB / Amp​

Answers

Answer 1

Answer:

the answer would be :


Related Questions

What is meant by the term primary producer in the food chain?​

Answers

Answer:

Hmm...

Explanation:

Think plants, grass, algae, etc. Base of the food chain.

[tex] \large{ \underline{ \bf{Primary \: Producers}}}[/tex]

They are autotrophic organisms which fix up the solar energy and manufacture their own food from inorganic material.They form the base of the food chain constituting first tropic level [tex] \sf (T_1) [/tex].Example: Phytoplankton, Grasses, Trees, etc.

where do faults normally happen

Answers

Answer:

This is what happens when the hanging wall rises relative to the footwall.

Explanation:

A boy sits half-way down a grassy slope. The force that keeps him
from sliding is
O Weight
O Friction
Gravity
O Momentum

Answers

Answer:

Friction is the answer.

i think it is b i am not sure

Explain how a plant gets and uses
energy

Answers

Answer:

it gets useful energy by the creation of sugar molecules through the process of Photosynthesis.

Explanation:

Sun water n soils in the groundnsjsksmsmskks

What are the different types of covalent bonds? Explain the characteristics of each, and how these types of bonds form.​

Answers

Question:What are the different types of covalent bonds? Explain the characteristics of each, and how these types of bonds form.​

answer:

Covalent bonds include single, double, and triple bonds and are composed of sigma and pi bonding interactions where 2, 4, or 6 electrons are shared respectively. Covalent compounds typically have lower melting and boiling points than ionic compounds. i hope this helps you i wish you a grate weekend and thanks giving


3. Explain the role of enzymes and how they affect the chemical reactions of living things.

Answers

Answer:

The role of enzymes is that enzymes are vital for life

Explanation:

Enzymes are vital because they are proteins that make the body work faster such as digestion and metabolism.

Enzymes are proteins that serve as biological catalysts by speeding up chemical processes. The enzyme is not destroyed during the reaction and is reused.

What do enzymes do ?

Enzymes help with the chemical reactions that keep a person alive and well. Many key functions in the body are supported by enzymes. Here are a few examples:

The digestive system: Enzymes assist the body break down more complex molecules, such as glucose, into smaller ones that may be used as fuel.DNA replication: DNA is found in every cell in the body. When a cell divides, it must duplicate its DNA. Enzymes aid in this process by unraveling the DNA coils.Toxins in the body are broken down by liver enzymes. To do this, it employs a variety of enzymes to aid in the process of eliminating the poisons.

Enzymes also aid in the following activities:

hormone manufacturingcell managementproducing movement to constrict the muscle producing movement to constrict the muscle transporting things around a cell respiration signal transduction

Hence, enzyme plays a vital role in functioning of the body.


Learn more about enzymes, here:
https://brainly.com/question/13878074


#SPJ5

The police know that the neighbor across from a house where a murder occurred sits on the porch nearly all day and believe he would have seen or heard something the night the murder took place. However, the neighbor doesn't want to testify. What can the prosecution rely on to get the neighbor to testify? A photo array A police sketch A subpoena Miranda rights

Answers

Answer:

A subpoena

Explanation:

Biology, I need help

Answers

Answer:

Photosynthesis

Explanation:

Photosynthesis is a process when plants or some microorganisms use the energy from the sun to produce glucose when water and carbon dioxide are present. The glucose is then converted into pyruvate that then releases ATP by cellular respiration. On the picture there is the sun that interacts with the cell that then makes the ATP

The phases of the Moon are caused by the...

Answers

Answer:

Earth

Explanation:

Half of the Moon is lit by the Sun while the other half is in darkness. The phases we see result from the angle the Moon makes with the Sun as viewed from Earth.

Answer:

Because of how the sun hits. So when the sun hits it the phases of the moon show.

Explanation:

fdggdsgdsg

Genotypes are written as 2 ______ representing each ______ for the trait

Answers

Answer:

letters, allele

Explanation:

The genotype is written as YY, Yy, or yy. The dominant allele is upper case (Y) and the recessive allele is lower case (y). Each letter represents one of the two alleles in the genotype.

Genotypes are written as 2 letters representing each allele for the trait.

What is genotype?

The genotype is an organism's genes; for one specific trait, we use two letters to represent the genotype.

A capital letter denotes the dominant form of a gene (allele), while a lowercase letter denotes the recessive form of the gene (allele).

In a bigger way, the term "genotype" recognized to an organism's genetic makeup; in other words, it describes an organism's entire set of genes. In a narrower sense, the term can refer to the alleles, or variant forms of a gene, that an organism carries.

From embryonic formation to adulthood, the genotype determines an individual's hereditary potentials and limitations.

Thus, there are two letters in genotype representing trait for each allele.

For more details regarding genotype, visit:

https://brainly.com/question/12116830

#SPJ5

Your skin is constantly producing new cells through cell division. How does this happen?

Answers

Answer:

This happens through interphase.

Check this answer for more info:

https://brainly.com/question/1757917

#LearnWithBrainly

Which statement does not describe radioactive atoms?
A. They are used for absolute dating of samples.
B. They change into atoms of a different element.
O C. They are extremely stable types of atoms.
D. Half of the atoms decay during one half-life.
SUBMIT

Answers

Answer:

B

Explanation:

B is the answer because if you put a different answer it won't make sence

Answer:

Its C on Ap.ex

Explanation:

If water has a density of 1g/cm will something with a density of 1 float or sink

Answers

Answer:it will float

Explanation:

this question is regarding climate change killing elephants​

Answers

Answer:

Explanation:

Climatology & Biology

Climatology (from Greek κλίμα, klima, "place, zone"; and -λογία, -logia) or climate science is the scientific study of climate, scientifically defined as weather conditions averaged over a period of time.

&

Biology is the natural science that studies life and living organisms, including their physical structure, chemical processes, molecular interactions, physiological mechanisms, development and evolution. Despite the complexity of the science, certain unifying concepts consolidate it into a single, coherent field.

So if we understand the way elephants work through biology then we can understand how change in temperature and weather can affect them

The picture shows a section of a wastewater treatment plant. This section of the plant removes oil and fats in water. What would be the purpose of blowing air into the wastewater?

Answers

Answer:The air forms a froth that floats on top and can be removed.

Explanation:

A mutation
A
is a person or an animal randomly selected from the population.

B
is a random change in a cell's DNA.

C
always occurs when the male's sperm joins the female's egg.

D
is produced by mating two different species.

Answers

Answer:

I'm pretty sure it's D. bc a mutation is something that's created from mixing two different species

Answer:

B (is a random change in a cell's DNA)

I just had this question

Explanation:

BRAINLIST PLEASE ANSWER

which one is conglomerate


Tilting,

Erosion,

Folding

Answers

Answer:

I think its erosion

Explanation:

Scare Monsters can be blue, purple or blue with purple spots. If we
cross a purple scare monster with a blue scare monster, what will be the
phenotypes of the offspring?

Answers

Answer:

Incomplete Dominance

Explanation:

Its incomplete dominance because if it has anything to do with colors blending to make anything new its always going to be incomplete dominance.

Which Earth processes break down rocks and transport the pieces? What types of rock can go through these Earth processes?

please help me its quarantine and i completely forgot LOL- call me dumb

Answers

Weathering/erosion and igneous rocks

What is your opinion about extrasensory perception? Do you think it exists? Explain your answer.

Answers

Answer:

I think it is true, I believe of this thing called "universal mind" I think extrasensory perception does exist because humans are already connected in ways that we cannot conceptualize right now, we just need to find some either technological or philosophical way to tap into it.

Explanation:

May I have brainliest please? :)

What are the three (3) main types of plate boundaries?

Answers

Answer:

Convergent boundaries: where two plates are colliding. Subduction zones occur when one or both of the tectonic plates are composed of oceanic crust.

Divergent boundaries – where two plates are moving apart. ...

Transform boundaries – where plates slide passed each other.

Explanation:

What are the causes of inherited genetic variation??
NEED NOW!!!!!!!!!!!!!!!

Answers

Answer:

What causes genetic variation?

Genetic variation can be caused by mutation (which can create entirely new alleles in a population), random mating, random fertilization, and recombination between homologous chromosomes during meiosis (which reshuffles alleles within an organism’s offspring).

Explanation:

PLZ mark brainliest also I hope this helps

Answer:

Genetic variation can be caused by mutation (which can create entirely new alleles in a population), random mating, random fertilization, and recombination between homologous chromosomes during meiosis (which reshuffles alleles within an organism's offspring).

Explanation:

Eye and body color in fruit flies are close together on the same chromosome.
What can be said about studying these traits?

A) The traits would not obey the law of segregation because they are always inherited separately.


B) The traits would not obey the law of independent assortment because they are always inherited separately.


C) The traits would obey the law of segregation because they are not inherited separately.


D) The traits would not obey the law of independent assortment because they are not inherited separately.

Answers

Answer:

C is the correct answer.

The traits would not obey the law of independent assortment because they are always inherited separately. Therefore, option (B) is correct.

What is The law of independent assortment?

The law of independent assortment is a fundamental principle in genetics that describes how genes are inherited independently of each other during meiosis. This principle was first proposed by Gregor Mendel, who discovered the basic laws of inheritance in pea plants.

According to the law of independent assortment, the inheritance of one trait is not affected by the inheritance of another trait, as long as they are located on different chromosomes or are far apart on the same chromosome.

During meiosis, homologous chromosomes pair up and line up randomly at the metaphase plate, and the orientation of one pair of chromosomes is independent of the orientation of another pair. This results in the independent assortment of genes, which allows for the formation of new genetic combinations and contributes to genetic diversity.

Learn more about The law of independent assortment, here:

https://brainly.com/question/29556097

#SPJ3

Several students were having a discussion about the role of chromosomes in inheriting traits.

Which statements made by the students provide the MOST accurate description?

[Select ALL that apply]

Chromosomes are the structures found in the nucleus of a cell. They are made from DNA, containing hereditary information in the form of genes that control how an organism will look and behave.

Genes contain thousands of chromosomes that carry specific information about building proteins for a particular trait.

Chromosomes are small sections of DNA that contain specific information about a trait to build proteins that people inherit. The thousands of different chromosomes passed from the parents allow for humans to look uniquely different.

Chromosomes come in homologous pairs (one from each parent) that each contain thousands of genes, determining traits expressed in the offspring.

Homologous chromosomes carry different gene sequences from each parent so the offspring may inherit more genes from one parent than they do from another parent.

Answers

Answer:

Chromosomes are the structures found in the nucleus of a cell. They are made from DNA, containing hereditary information in the form of genes that control how an organism will look and behave.  

Chromosomes come in homologous pairs (one from each parent) that each contain thousands of genes, determining traits expressed in the offspring.

Explanation:

Chromosomes are the structures found in the nucleus of a cell. They are made from DNA, containing hereditary information in the form of genes that control how an organism will look and behave.  - this is true. Prior to cell division, DNA molecules are organized into large structures called chromosomes. Specific regions of a DNA molecule are called genes. These dictate specific proteins which control our traits.

Genes contain thousands of chromosomes that carry specific information about building proteins for a particular trait.  - this is false - genes are segments of DNA that control specific traits by dictating the structure and functions of proteins. Chromosomes contain thousands of genes

Chromosomes are small sections of DNA that contain specific information about a trait to build proteins that people inherit. The thousands of different chromosomes passed from the parents allow for humans to look uniquely different. - this is false - chromosomes are large structures, genes are the relatively small sections of DNA. Humans have 23 pairs of chromosomes, not thousands.

Chromosomes come in homologous pairs (one from each parent) that each contain thousands of genes, determining traits expressed in the offspring.  - this is true. In diploid organisms, like humans, have two copies of each chromosome. These chromosomes contain slightly different versions of genes, which make us unique.

please please please need the answer ASAP. :)) Which of the following is NOT a function of bone?


storage of calcium and fat

exchange oxygen and carbon dioxide

protection

support and movement

making blood

Answers

Answer:

exchange oxygen and carbon dioxide

Explanation:

that is the respiratory system

pls mark brainliest :) <33

Identify the order of the eight levels of taxonomy, from least specific (largest) to most specific (smallest), using the drop down menus.

Answers

Kkkkkkkkkkkkkkkkkkkkkkkk

Answer:

✔ Level 6

Family

✔ Level 7

Genus

✔ Level 2

Kingdom

✔ Level 1

Domain

✔ Level 4

Class

✔ Level 8

Species

✔ Level 5

Order

✔ Level 3

Phylum

Explanation:

What characteristic of metals makes them able to conduct electricity so easily?

They are willing to give up their valence electrons.

Their nucleus has more protons than neutrons.

They are malleable.

They have a simpler electron arrangement.

Answers

Answer:

I can answer with your option here my answer

Explanation:

The structure and bonding of metals explains their properties : They are electrical conductors because their delocalised electrons carry electrical charge through the metal. They are good conductors of thermal energy because their delocalised electrons transfer energy. They have a simpler electron arrangement.

a more simple answer is (A)

"They are willing to give up their valence electrons."

took the test, hope this helps :)

What are the products when lipase acts on its substrate?

Answers

Answer:

Explanation:

Lipase is a type of enzyme known as a hydrolase and is responsible for catalysing the hydrolysis of triglycerides (the substrate) into fatty acids and glycerol

What part of your nervous system gathers information for the brain to translate into response?

A. Sensory organs

B. Spinal cord

C. Glands

Answers

Answer: sensory glands I think

is CaBr2 ionic or covalent?

Answers

Answer: Covalent I’m pretty sure.

Ionic hope it helps
Other Questions
Galileo finds evidence that Earth is not the center of the universe. This is a level of organization diagram.Which term does row x represent? Im not good at Spanish but,Yo esta una decepcinCan Spanish people translate to the ones who dont understand? An embryo created by male-female reproduction has traits from _____. A) The male only B) Both the male and the female C) The female onlyPlz hellp! 20 points! help answer asap will mark brainliest to first person if i can Richard McCarthy (born 2/14/64; Social Security number 100-10-9090) and Christine McCarthy (born 6/1/1966; Social security number 101-21-3434) have a 19-year-old son (born 10/2/99 Social Security number 555-55-1212), Jack who is a full-time student at the University of Key West. The McCarthys also have a 12-year-old daughter (Social Security number 444-23-1212), Justine, that lives with them. Richard is the CEO at a national paper company. His Form W-2 is provided. NEED HELP WILL MARK BRAINLYIST PLEASE!!!!!! please answer I need the answer now, please Ellen plans to leave an 18 percent tip with her $23.75 lunch bill. Which of these expressions could she use to determine her total bill? Check all that apply.Ellen plans to leave an 18 percent tip with her $23.75 lunch bill. Which of these expressions could she use to determine her total bill? Check all that apply. if jogging burns on average 140 calories every 15 minutes. how many calories are you burning in one hour. You and your friend skate at the same rate.You complete 5 laps in 7 minutes.How long does your friend take to complete 15 laps? what is the value of f(x) when x= -3 A _________is a dream that you are dedicated to; therefore, you plan and work for it. A. Decision B. Action plan C. Goal D. Lifestyle factor Help please Hurry!!! quick someone make me a poem with these words please and thank you p.s use 3 words that's in the picture Jamie did some push-ups on Monday and Tuesday. On Tuesday, he did 300 push-ups, which was three times the difference of the number of push-ups he did on Monday and the number 50. How many push-ups did he do on Monday?Define the variable 1. Suppose that during its flight, the elevation e (in feet) of a certain airplane and its timetin minutes since takeoff, are related by a linear equation. Consider the graph of thisequation, with time represented on the horizontal axis and elevation on the verticalaxis. For each situation, decide if the slope is positive, zero, or negative.a. The plane is cruising at an altitude of 37,000 feet above sea level.b. The plane is descending at rate of 1000 feet per minute.c. The plane is ascending at a rate of 2000 feet per minute. One and a half hours before practice or a game you should...eat a light snack.drink fruit juice.drink water.eat a full meal. If you buy a radio that cost $860 and sales tax is 6%. Find the total price you would pay. Math assignment cant get it