In the cell bio lab, we use company manufactured gels, however you can make you own polyacrylamide gels. List all of the ingredients found in an SDS-PAGE gel. Which ingredients are responsible for polymerizing the solution?

Answer:

The list of ingredients are used in this method are as follow-

1. Acrylamide - mostly 30%

2. Bisacrylamide - mostly 0.8%

3. 1.5M Tris-Hcl pH 6.8 for stacking gel and 8.8 for separating gel.

4. 10% SDS

5. 10% APS

6. TEMED

7. Double distilled water

Polymerization:

Acrylamide is a polymer that is used to dissolved in buffer and bisacrylamide is the cross linking agen.

Two types of gels are prepared for separation of protein. Stacking gel is usually 4 or 6% and separating gel is usually 10 to 20%. Lower concentration of stacking gel will allow the migration of all the protein same because of larger pore size. It will stack or line up the proteins for its next level of separation.

Higher concentration of separating gel will provide resistance to the movement of protein because of the low pore size and therefore linear bands can be achieved neatly.

Using the field of view calculated in Exercise 1 for the high power lens, what is the approximate diameter of each of the cells in the Bacteria Coccus Form slide in Photo 10? Show your calculations.
400x Magnification
0.4 mm field view

Transfusion of type AB blood would introduce both A and B ______________ which would be ______________ monosaccharides type

What is a community?
1 all the animals that live in a habitat
2 a single species that lives in a habitat
3 all the species that live in a habitat
4 a population that lives in a single habitat

A population of lab rats recently escaped into the sewer system of Washington, D.C. A brave researcher sampled this population and estimated the following phenotypic frequencies (which were caused by a single gene with two alleles: E and e).
61% normal eyes (EE)
26% cross-eyed (Ee)
13% blind (ee)
If this population were to reach a Hardy-Weinberg Equilibrium (HWE) in one generation, what would the phenotypic frequencies be in that new HWE generation? Let freq (e)=p and freq (e)=q. (Tip first calculate p and q from the observed, and provided, phenotypic frequencies)
freq EE =
freq Ee =
freq ee =

explain how typical cells are adapted to their function​

The immunoglobulin light chain does not have a D segment. In humans, there are two different possible light chain proteins, termed kappa and lambda. Assume that there are 35 V segments and 5 J segments in the human kappa light chain region, and there are 30 V segments and 4 J segments in the human lambda light chain region. How many possible light chain genes can be created from these different segments, simply due to combinatorial diversity

Need answer ASAP! A car runs out of gas and slowly coasts to a stop. This is an example of the following?
O A. short time, small force
B. long time, small force
O c. short time, large force
D. long time, large force

A stem cell is
A. A specialized cell that can divide limitlessly but cant differentiate
B. A specialized cell that can divide limitlessly.
C. An unspecialized cell that cant divide limitlessly nor differentiate into specialized cell.

all dicots are annuals true or false​

In terms of the hierarchical scales of biological organization, __1__ are a very large scale, __2__ are a very small scale, and __3__ is between them.

how do the decisons that we make each day influence how efficiently our body operates

Match the following Rose Water hyacinth Hydrilla Lotus Pine trees Mango trees A non-flowering plant A flowering plant A floating plant A underwater plant Hot and plain place Cold places Name the following​

Linda received the flu vaccine this year.

Which of the following statements is true?


Linda's T-cells will produce memory cells against the virus.


Linda's body will produce antigens against the flu virus.


Linda's immune system will produce lifelong immunity against any form of the flu.


Linda's immune system will produce antibodies against the flu virus.

Once the neural plate edges have fused to become a neural tube, the tissues that were at the edges of the plate now form the __________ region the neural tube. Fill in the blank.

why is meiosis called reduction division?​

Hello.
All of the DNA in the nucleus of a cell can potentially be mutated?

True or false?

I will give brainly

biology chapter 33 assessment 972

The history of life has been punctuated by several mass extinctions. For example, the impact of a meteorite may have wiped out most of the dinosaurs and many forms of marine life at the end of the Cretaceous period. Fossils indicate that plants were less severely affected by this mass extinction. What adaptations may have enabled plants to withstand this disaster better than animals

Salt minerals formed by which method

Scenario 1:

Genetic engineering can be used to create more productive strains of farm animals used for milk and meat production. By adding genes to an animal’s DNA, the animal can be made to be more resistant to common infections. This can reduce the need to administer large doses of antibiotics to the animals.

Do you think that this type of genetic engineering should be pursued? Explain your answer. (5 points)

What are some possible impacts (positive and negative) of this type of genetic engineering on individuals, society, and the environment? (5 points)


Scenario 2:

Mike was adopted, and his biological family history is unknown. Although he is healthy, he would like some understanding of his genetic makeup, including potential health risks and genes that he could pass on to his children. Mike has heard about commercial laboratories that can compare segments of your DNA to those of people with common hereditary diseases in order to give you some idea of how susceptible you are to the diseases. The results of these types of tests are highly inconclusive. If Mike’s DNA showed that he shares similarities in a segment of DNA with people who have a given disease, his chances of developing that disease or passing it on to his children may be slightly elevated, but they are not 100 percent. These tests can cost more than $2,500 and are not covered by many insurance companies.

Do you think that Mike should undergo the genetic tests? Explain your answer. (2 points)

What are potential pros and cons of having such tests done? (5 points)

Describe how the availability of these genetic tests might affect the frequency of genetic diseases in individuals and populations. (3 points)

Acidic amino acids have two -COOH and one -NH2 group per molecule. Select the pair that consists of acidic amino acid
a) Aspartic Acid , Glutamic Acid
b) Lysine , arginine
c) Glycine and alanine
d) Both a and b​

On a plant, stomates are small openings that regulate the passage of air and water vapor to and from the plant. Generally, the stomates open during sunlight and exchange gases with the atmosphere. The stomates of a few specialized desert plants can open only at night. The best explanation for the pattern of stomate opening and closing is that the desert plants are adapted to protect against
(1) harmful insects
(2) dust particles
(3) destructive plant disease
(4) excess water loss
(5) total energy loss

Please help me somebody answer this.
​

In classical conditioning _____ is the unlearned, naturally occurring response to the unconditioned stimulus (US). (Pyscology)

If you were given the power to control the United States government and policy what changes would you make regarding the environment and how would you fund these? You can consider your response to the question and/or include other issues.

The mutation of a cell for a specific function is called

The toxins responsible for botulism are proteins that inhibit the release of acetylcholine, which in turn can cause muscle paralysis. In extreme cases the paralysis can involve the respiratory muscles, and people can die of respiratory failure. This mechanisms of action indicates that the toxins are

What is a difference between complete metamorphosis and incomplete metamorphosis?

Two stages of a butterfly's life cyles are described below.

Stage 1: The caterpillar sheds its skin as it grows.
Stage 2: The butterfly breaks out of the chrysalis.

Which statement is true about the stages?

What determines the temperature of a layer of the atmosphere?
A. The way thlmayer interacts with the layer below it
B. The average wind speed in the layer
c. The way the layer interacts with solar radiation
D. The altitude of the solar radiation

Which of the following is the best definition of climate?

1 . The day to day changes in the atmosphere

2. The average weather over a long period of time and over large areas

3. The state of the atmosphere across the world

The large number of alveoli in lungs improve gas exchange by ________. increasing the surface area for diffusion decreasing the distance for diffusion changing the diffusion constant increasing the concentration difference