In a separate maddyase experiment using (Et)-10nM, the reaction velocity is measured as 3uMS. What is the substrate concentration used in the experiment

Answers

Answer 1

Answer: hello your question has some missing data attached below is the missing data

Applying the Michaelis-Menten Equation

An enzyme is found that catalyzes the reaction X ⇋ Y Researchers find that kcat for this enzyme is 600s^-1 and carry out several additional experiments.

answer :

substrate concentration [ x ] = 10⁻⁵ M

Explanation:

Given data:

[Et ] = 10 * 10⁻⁹ M ,  

Reaction velocity ( V₀ ) = 3* 10⁻⁶ Ms⁻¹

applying Michaelis-Menten  Equation

= 3* 10⁻⁶ = [tex]\frac{600 *10*10^{-9} * (X) }{10^{-5} + (X) }[/tex]  ,    

= ( 3 * 10⁻⁶ )( x ) + ( 3 * 10⁻¹¹ ) = ( 6 * 10⁻⁶ ) ( x )

hence the substrate concentration [ x ] = 10⁻⁵ M


Related Questions

describe the role of enzymes in seeds germination​

Answers

During germination, seeds use sugars and other molecules as a substrate for respiration. α-amylase and β-amylase are involved in degradation of endosperm starch. Starch hydrolysis into glucose is catalyzed by action of α- and β-amylases, debranching enzyme and α-glucosidases (maltase)

Is the Use of energy to move particles against the concentration gradient

Answers

Answer:

During active transport, substances move against the concentration gradient, from an area of low concentration to an area of high concentration. This process is “active” because it requires the use of energy (usually in the form of ATP). It is the opposite of passive transport.

Sometimes the most practical way to do an experiment is not to perform it in an animal, but to look at cell responses in culture. Cells from mice, humans, and other mammals have been used to establish cell culture lines that have been very important for research. You are studying a novel water-soluble mouse hormone. You know cell culture can be a practical model to reveal protein function, so you apply the hormone to yeast cells, but nothing happens. What is a likely explanation for why nothing happened in your experiment

Answers

Answer:

Yeast cells likely do not have receptors that bind to this specific water-soluble mouse hormone

Explanation:

Receptors are molecules capable of binding specific signaling molecules (i.e., ligands). For example, steroid receptors can only bind to specific steroid hormones (e.g. estrogen, progesterone). Plasma membrane receptors are protein receptors that bind to water-soluble ligands. These receptors are embedded in the cell plasma membrane and usually contain several transmembrane domains. Examples of cell membrane receptors include G protein-coupled receptors (GPCRs), enzyme-linked protein receptors and ion channel receptors.

describe the stages in the process of selective breeding​

Answers

Answer:

Selective breeding takes place over many generations. These are the main steps for both plants and animals involve:

Decide which characteristics are important enough to select.Choose parents that show these characteristics from a mixed population. They are bred together.Choose the best offspring with the desired characteristics to produce the next generation.Repeat the process continuously over many generations, until all offspring show the desired characteristics.

Hope this answer helps you..!!

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.

Select this answer as the BRAINLIEST!!

Answer:

I think the answer is Selective breeding takes place over many generations. These are the main steps for both plants and animals involve:

Decide which characteristics are important enough to select.

Choose parents that show these characteristics from a mixed population. They are bred together.

Choose the best offspring with the desired characteristics to produce the next generation.

Repeat the process continuously over many generations, until all offspring show the desired characteristics.

Hope this answer helps you ^^

Why is it essential for SARS-CoV2 RNA be translated on ribosomes before it can be replicated?

Answers

The SARS-CoV-2 non-structural protein 1 (Nsp1), also referred to as the host shutoff factor, suppresses host innate immune functions. By combining cryo-electron microscopy and biochemistry, we show that SARS-CoV-2 Nsp1 binds to the human 40S subunit in ribosomal complexes, including the 43S pre-initiation complex and the non-translating 80S ribosome. The protein inserts its C-terminal domain into the mRNA channel, where it interferes with mRNA binding. We observe translation inhibition in the presence of Nsp1 in an in vitro translation system and in human cells.

select the correct answer from each drop down menu.
both anaerobic and aerobic respiration use __ to produce ATP and NADH

Answers

I dont see the answers for the drop down menu

What are threatened species?Question 1 options: species we do not have enough data for to determine their population numbers only large mammals like elephants, pandas, and whales only species poached for their organs or other parts species at risk for extinction species that have already gone extinct

Answers

Answer:

The correct answer is - species at risk for extinction.

Explanation:

Extinction is a threat to species of any living organism. The death or removal of last individual of a species is the condition of extinction.

It is commonly referred to the species as mostly species extinct from earth. Any size or type of species of living organism can be extinct.

In plants, most differentiated cells retain Group of answer choices a complete set of their genes and retain the ability to express those genes under certain circumstances. a complete set of their genes but lose the ability to express most of those genes. only a tiny fraction of their original set of genes. the ability to dedifferentiate but then cannot return to their original differentiated state.

Answers

Answer:

due to their excessive numbers in tissues. ... because their developmental potential is limited to certain tissues. In plants, most differentiated cells retain. A) only a tiny fraction of their original set of genes.

Explanation:

hmm i think this is your answer

The image of a scientist collecting a sediment core was taken at the Wild Harbor salt march on Cape Cod, Massachusetts. What evidence is this person hoping to collect from this research?
Scientist taking a sample of marsh soil.
© 2016 Woods Hole Oceanographic Institution


She hopes to discover if the oil is still floating on the surface.
She hopes to see if the oil is still present deep down below the surface.
She hopes to collect animals that have died from the spill.
She hopes to see how easily the oil can be removed from the soil.

Answers

Answer:

A. She hopes to discover if the oil is still floating on the surface.

Give the functions of the following parts of the respiratory system - Nostril - trachea - bronchiole - alveoli - pharynx - larynx - lungs  - ribs - diaphragm                                                                   

Answers

Answer:

Respiratory System: The respiratory system's main job is to move fresh air into your body while removing waste gases.

Nostril: To warm air on inhalation and remove moisture on exhalation.

trachea: Serves as passage for air, moistens and warms it while it passes into the lungs, and protects the respiratory surface from an accumulation of foreign particles.

bronchiole: To deliver air to a diffuse network of around 300 million alveoli in the lungs.

alveoli: To exchange oxygen and carbon dioxide molecules to and from the bloodstream during breathing.

pharynx: Helps the respiratory system by allowing air to make its way to the respiratory tract.

larynx: Produces vocal sounds and prevents the passage of food and other foreign particles into the lower respiratory tracts.

lungs: To help oxygen from the air we breathe enter the red cells in the blood.

ribs: To aid respiration and help protect the lungs.

diaphragm: Contracts and flattens when you inhale. Creates a vacuum effect that pulls air into the lungs, and relaxes when exhaling.

nitrogenous base + sugar + phosphate group =

Answers

Answer:

Nucleoside

Explanation:

Nucleotide is an organic compound which is made from of nitrogenous base, a sugar, and a phosphate group.

Answer: nucleotide

Each nucleotide is composed of a nitrogenous base, a five-carbon sugar (deoxyribose), and a phosphate group. There are four nitrogenous bases in DNA, two purines (adenine and guanine) and two pyrimidines (cytosine and thymine). A DNA molecule is composed of two strands.

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Which of the following is TRUE about osmosis?
i. Osmosis is a type of active transport.
ii. Kidney dialysis is an example of osmosis process.

Answers

ii is true about osmosis

what are hereditary materials​

Answers

Answer:

DNA, or deoxyribonucleic acid, is the hereditary material in humans and almost all other organisms. Nearly every cell in a person's body has the same DNA.

Answer:

DNA, or deoxyribonucleic acid, is the hereditary material in humans and almost all other organisms. Nearly every cell in a person's body has the same DNA.19-Jan-2021

In the scientific process, what is the correct sequence for the following steps: A: Recognizing a problem/Asking a question; B) Testing a hypothesis; C Drawing inferences
Group of answer choices

ABC

ACB

CAB

BCA

Answers

Answer:

ABC

Explanation:

Ask questions, make a hypothesis, test the hypothesis, analyze results, draw conclusions, communicate results.

define and describe osmosis​

Answers

Answer:

Movement of a solvent (such as water) into a solution with a higher solvent concentration by means of a semi-permeable membrane (as from a live cell), which tends to equal solute concentrations in the membrane on both sides.

Explanation:

Osmosis, the spontaneous transition, distribution, or diffusion of water and other solvents over a semipermeable membrane. In 1877, a German physiologist, Wilhelm Pfeffer, researched the process, which is important in biology.

Osmosis is the passage of water down its concentration gradient, across a semi-permeable membrane. An everyday example is a plastic wrap in your kitchen: it allows air and water vapor to move across it, but no water or food. The membranes of cells are semi-permeable, too.

Give a reason why lack of roughage in diet often lead to constipation?​

Answers

Answer:

Roughages increase fecal volume and promote peristalsis

Explanation:

Constipation refers to the condition in which bowel evacuations occur infrequently, leading to health problems. Some of the most common causes of constipation include 1-insufficient intake of dietary fibers or roughages, 2-poor hydration, 3-lack of physical exercise, 4-abuse of laxatives, etc. Dietary fibers are divided into two classes based on their water solubility: soluble and insoluble. These fibers represent the part of plant foods that the body can't digest, which absorbs and retains water (soluble fibers) as foods pass through the bowel and create bulk (insoluble fibers), thereby leading to regular bowel habits.

What type of unconformity did Hutton see at Siccar Point and how was this uniformity formed?

Answers

Answer:

Hutton's Unconformity

At Siccar Point, during the lower Silurian Llandovery epoch around 435 million years ago, thin beds of fine-grained mudstone were laid down gradually deep in the Iapetus Ocean, alternating with thicker layers of hard greywacke formed when torrents swept unsorted sandstone down the continental slope. Siccar Point is a rocky promontory in the county of Berwickshire on the east coast of Scotland. It is famous in the history of geology for Hutton's Unconformity found in 1788, which James Hutton regarded as conclusive proof of his uniformitarian theory of geological development.

If an individual has trisomy resulting in Down syndrome and has an extra chromosome in each cell, what mutation must have occurred?
A. A nondisjunction mutation in the gamete of one of the parents
B. A nondisjunction in both gametes of the parents
C. A nondisjunction in one of the first gamete cells of the individual
D. A nondisjunction mutation in body cells of both parents

Answers

Answer:

if  An individual has an extra chromosome in every one of its cells, the mutation that must have occurred is A non-disjunction mutation in the gamete of one of the parents

If an individual has trisomy resulting in Down syndrome and has an extra chromosome in each cell, the mutation that must have occurred is A. A nondisjunction mutation in the gamete of one of the parents.

Down syndrome simply means a genetic disorder that is caused when there's an abnormal cell division that then leads to extra genetic material.

The effect of Down syndrome is the fact that it leads to an intellectual disability, developmental delays, and a distinct facial appearance.

The mutation that has occurred with someone that has a Down syndrome is the nondisjunction mutation in the gamete of one of the parents.

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Which of these factors would most likely strengthen the effectiveness of

Answers

Answer:

Which of these factors would most likely strengthen the effectiveness of CITES?

Answer: Funding for on-the-ground enforcement

According to the graph, which region is expected to reach a population of 50 million people in the year 2040?​

Answers

The correct answer to your question is A. :)

According to the graph, the regions that are expected to reach a population of 50 million people in the year 2040 are (A). Mexico, Central America, the Caribbean island, and South America.

What is a population graph?

A population graph is a type of graph that represents the distribution of population, increase or decrease of population of different areas or countries.

This population graph shows the population of different countries, some countries already reached 50 million. The countries which will be reached are in the purple line on the graph that are Mexico, Central America, the Caribbean island, and South America.

Thus, option (A), Mexico, Central America, the Caribbean island, and South America will have 50 million people in the year 2040.

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How do membrane proteins contribute to the membrane potential?

Answers

Answer:

The proton pump results....

Explanation:

double checked my notes

Aquaporins result in a combination of positive charges intracellularly and negative charges extracellularly

What are Aquaporins?

Aquaporin is a group of membrane proteins found in cell membranes and selectively permit water to pass into and out of the cell. Also known as water channels, aquaporins are integral membrane pore proteins. Aquaporins are found in plants, bacteria and animals. They are observed in the vacuolar membranes and plasma membranes. The basic function of most aquaporin is to transport water across cell membranes in response to osmotic gradients created by active solute transport. Aquaporins enable passive transport which means they allow the movement of molecules across a cell without using energy.

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Complete the following statement using the choices below. Air moves out of the lungs when the pressure inside the lungs is ________. less than the pressure in the atmosphere greater than the intra-alveolar pressure greater than the pressure in the atmosphere equal to the pressure in the atmosphere

Answers

Answer:

Air moves out of the lungs when the pressure in the lungs is greater than the pressure in the atmosphere.

Explica brevemente en que consiste la teoría de la deriva continental y menciona los contenientes que se crearon a partir de la Pangea

Answers

Esta teoría hace referencia al movimiento actual de las placas que sustentan los continentes y que se desplazan a lo largo de millones de años. En toda la historia geológica de la Tierra, los continentes no han estado siempre en la misma posición. Existe una serie de evidencias que veremos posteriormente que ayudaron a Wegener a refutar su teoría. El movimiento se debe a que continuamente está formándose nuevo material procedente del manto. Este material se crea en la corteza oceánica

What is the function of the enzyme RNA polymerase during transcription?
helps slow down the process of transcription
helps speed up the process of transcription
aids in destroying the mRNA sequence that was read
aids in packaging amino acids for use at a later time

Answers

Answer:

The correct answer is - helps speed up the process of transcription.

Explanation:

RNA polymerase binds to the DNA sequence and copy a single DNA sequence into RNA and helps a single gene to produce hundreds or even thousands of RNA molecules during transcription.

It helps in the speed up the transcription by copying the sequence and producing mRNA for translation process later.

Answer:

I hope this helps.

Explanation:

Plants and algae most recently shared a common ancestor 480 million years ago, and plants colonized land shortly after that. Light, water, minerals, and dissolved gases surround the whole body of a submerged green alga, and the buoyancy of water provides physical support. What part did plants evolve to absorb water and minerals on dry land

Answers

Answer:

vascular tissues

Which equation represents fertilization?
haploid + diploid = diploid
diploid + haploid = haploid
haploid + haploid = diploid
diploid + diploid = diploid

Answers

Answer:

the answer is (C) haploid + haploid = diploid

Explanation:

edge 2021

Haploid+ Haploid = Diploid represents fertilization. So, the correct option is (C).

What is Fertilization?

Fertilization is defined as the process in which two gametes where one male and one female first form a zygote and then lead to the development of an embryo.

These germ cells are cells that give rise to gametes – either egg cells that are female gametes or sperm cells that are male gametes.

These germ cells are the only cells that can undergo both mitosis and meiosis. These are the diploid cells present in the gonads. After meiosis, haploid gametes are produced. In fertilization two haploid gametes fuse (n+n) and form a diploid zygote (2n)

Thu, Haploid+ Haploid = Diploid represents fertilization. So, the correct option is (C).

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Science is not a process, but just a body of facts that
accumulates overtime.
TRUE
FALSE

Answers

Answer:

False

Explanation:

Science is more about gaining knowledge than it is about simply having knowledge. Science is a way of learning about the natural world that is based on evidence and logic. In other words, science is a process, not just a body of facts. Through the process of science, our knowledge of the world advances.

Propose advantages and disadvantages of nuclear energy as an energy source.

Answers

Answer:

Advantages:

Produces no polluting gases.

Does not contribute to global warming.

Very low fuel costs.

Low fuel quantity reduces mining and transportation effects on environment.

High technology research required benefits other industries.

Disadvantages:

Waste is radioactive and safe disposal is very difficult and expensive.

Local thermal pollution from wastewater affects marine life.

Public perception of nuclear power is negative.

Costs of building and safely decommissioning are very high.

Cannot react quickly to changes in electricity demand.

what do you mean by components of mixture?​

Answers

Answer:

The components of a mixture are the items that are mixed together.

Which of the following factors does not affect soil formation?
a. precipitation
b. time
C. erosion
d. none of the above
Please select the best answer from the choices provided
B
Ο Ο Ο Ο
PLS HELP ASAP

Answers

Erosion EASY precipitation effects soil so does time the longer the more it changes

Answer:

b is the answer

Explanation:

edge2021

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