Genetic engineering involves various stages.

1 human DNA is inserted into bacterial plasmid DNA
2 recombinant plasmid inserted into bacteria
3 restriction enzyme cuts bacterial plasmid DNA
4 restriction enzyme cuts human DNA

What is the correct sequence for genetic engineering?​

Answers

Answer 1

Answer:

4, 3, 1, 2

Explanation:

FIRST: cDNA is synthesized to be used as a gene of interest: processed (spliced) mRNA (no introns) is reverse transcribed to cDNA using reverse transcriptase, an enzyme from retroviruses

SECOND: Restriction enzyme used to cut GOI

THIRD: Same Restriction enzyme used to cut plasmid (sticky ends are created)

FOURTH: GOI connects/hybridizes with plasmid due to base-pairing

FIFTH: DNA ligase connects the recombinant DNA strands


Related Questions

Question 6 of 25
Which is an example of a vestigial structure?
A. Raccoon tail
B. Fish gills
C. Whale pelvis bone
D. Human arm
help:,)

Answers

the answer for your question is D

What happens during S phase?

O A. Chromosomes are duplicated.

O B. DNA separates into two nuclei.

O C. The cell splits in two.

O D. Cytoplasm is manufactured.

Answers

Answer: A. Chromosomes are duplicated.

Explanation: This is the correct answer on the quiz.

Chromosomes are duplicated in S phase. Therefore, option (A) is correct.

What do you mean by S phase?

Between G1 phase and G2 phase, DNA replication takes place during S phase of the cell cycle. The processes that take place during S-phase are tightly controlled and highly conserved.

The cell replicates its genetic material entirely during the S phase of DNA synthesis; At the end of S phase, a normal diploid somatic cell with a DNA complement of 2N acquires a DNA complement of 4N.

DNA synthesis or replication occurs during the S phase of the cell cycle, which occurs prior to the interphase. Before entering mitosis or meiosis, the cell's genetic material is replicated in this manner, leaving enough DNA for daughter cells to divide.

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latex from _______ is the source of biodiesel
A. Jatropa
B. Tulasi
C. Neem
D. Cactus​

Answers

Latex from cactus is the source of biodiesel.

Heather is doing an acid-base reaction. She has 25.00 mL of hydrochloric acid of unknown concentration in a flask. She wants to add a basic solution to the acid drop by drop, until all the acid has reacted.

Answers

Answer:

lwlwlelpepwpspsppa

Explanation:

ookeieorooroekrkrkrklrkdkdodkekiekeieie

what is the answer please help

Answers

Answer:

never become laws

Explanation:

trust

What is the Defenition of Metabolism?

a
When offspring change and develop over millions of years
b
When living things change over time.
c
The flow of chemical energy in an organism.
d
When an organism produces an offspring

Answers

The answer is

C. The flow of chemical energy in an organism.

Metabolism is the chemical processes that occur within a living organism in order to maintain life.

What is the Dependent Variable?

Answers

Answer:

pitcher with the same velocity

Which of the following does NOT secrete hormones?

A. Ovaries
B. Skin
C. Adrenals
D. Pancreas

Answers

Answer:

skin acts as a protective barrier, therefore it does not secrete hormones

Skin does not secrete hormones. Thus, the correct option is B.

What are Hormones?

Hormones may be defined as chemical substances that are produced by the organ system of an organism and transported at the site of action via the bloodstream. Hormones act as messenger molecules in the body.

Skin acts as the primary or first line of defense for the immune system. It does not secrete any hormones. It plays an important role in innate immunity. Ovaries are responsible for the secretion of hormones like estrogen and progesterone in females and regulate all the prospective consequences in the body of females.

The adrenal gland secretes hormones like cortisol and aldosterone which are steroid hormones. Such hormones are important in regulating metabolism, inflammation, immune functions, etc. The Pancreas secretes insulin and glucagon which play an active role in regulating the level of glucose in the blood.

Therefore, the skin does not secrete hormones. Thus, the correct option is B.

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How fast does E. coli manifest symptoms A. 3 mins B. 3 hours C. 3 days D. 3 weeks

Answers

Answer:

C. 3 days

Explanation:

Answer:

c

Explanation:

2-5 days after exposure is when symptoms show

taken from the minnesota department of health

Cells in the human body carry out different functions. Which cells help the body fight off diseases and remain healthy? Choose the two that apply.

Answers

Phagocytes and lymphocytes

2. Sam and Jerry are having a discussion in their Environmental Science lass. Sam
says that abiotic factors determine what biotic factors can be in an ecosystem. Jerry
says that biotic factors determine what abiotic factors can be in an ecosystem. Who
is correct? Use an example to support your answer. (2 points)

Answers

Answer:

Jerry is right.

Explanation:

Abiotic factors can kill biotic factors, so technically Jerry is correct, just think of it as a TDI game, let's say Chris is an Abiotic factor, and the campers are biotic factors, Chris chooses weather or not they stay.

_____ are related to poor productivity of harvest due to poor care for soil.

Answers

Answer:

Soil Tillage, Climate Change and Soil Carbon Sequestration

Explanation:

In a rainforest, there are trees of different sizes as shown in the figure below.
The trees in the UNDERGROWTH layer will probably face competition for which of the following resources?

a. Air
b. Water
c. Animals
d. Sunlight

Answers

Animals is the correct answer hope this helps

PLEASE I NEED HELP ASAP GIVING 75 POINTS IF YOU COMPLETE ALL AND BRAINLIEST!!!
Which type of electromagnetic radiation has more energy than visible light and can pass through the human body?
A. X-rays
B. radio waves
C. ultraviolet light
D. microwaves
2. Which information about objects in space can spectroscopy provide? Select the two correct answers.
A. timing of solar flares
B. size
C. composition
D. temperature
3. Which is electromagnetic radiation with wavelengths between 380 nm and 700 nm?
A. ultraviolet light
B. microwaves
C. radio waves
D. visible light
4. Which are examples of non-visible light?
A. rainbows and X-rays
B. radio waves and ultraviolet light
C. white light from a lightbulb and rainbows
D. white light from the sun and microwaves
5.What does it mean when an emission line of helium from a galaxy is shifted toward red?
A. The galaxy is moving away from the viewer.
B. The galaxy is moving toward the viewer.
C. The galaxy contains a large percent of hydrogen.
D. The galaxy contains a small percent of hydrogen.

Answers

Answer:

Which type of electromagnetic radiation has more energy than visible light and can pass through the human body?

answer 1 = A Xrays

answer 2= temperature,timing of solar flares

answer 3= visible light

answer 4=ultraviolet light, radio waves

pls make me brainliest

Explanation:

1. X-ray type electromagnetic radiation has higher energy than visible light and can pass through the human body.

2. Spectroscopy can provide composition and temperature information about objects in space.

3. Visible light is electromagnetic radiation with wavelengths between 380 nm and 700 nm.

4. Radio waves and ultraviolet light are examples of non-visible light.

5. When the emission line of helium from the galaxy is red-shifted as the galaxy is moving away from the viewer

The correct options for 1, 2, 3, 4 and 5 are A, (C and D), D, B and A respectively.

X-rays are valuable in medical imaging and other uses because they have more energy than visible light and can penetrate the human body.

By examining the light emitted or absorbed by the object, which forms a characteristic spectral pattern, spectroscopy can provide details about the composition and temperature of objects in space.

The human eye can perceive visible light because its wavelengths, which range from 380 nanometers to 700 nanometers, fall within this range.

Since their wavelengths lie outside the visible spectrum, radio waves and ultraviolet light are two examples of non visible light.

A galaxy is said to be moving away from the observer when the helium emission line from that galaxy is red-shifted in its spectrum. The Doppler effect, in which light emitted from objects moving away from the observer appears to be skewed toward longer (reddish) wavelengths, is what causes this redshift. This event is an important piece of evidence for the Big Bang theory and the expansion of the universe.

Therefore, the correct options for 1, 2, 3, 4 and 5 are A, (C and D), D, B and A respectively.

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suggest TWO reasons why fewer birds and other small animals can survive in the areas left after trees have been cut down​

Answers

Answer:

One is that they migrate to more fertile places.

Second, some animals may die because they can't stand the conditions of treelessness. But some animals can survive. because of adaptation

Explanation:

Removal of trees and other types of vegetation can reduce the availability of food, shelter and breeding habitat

What is Deforestation?

Deforestation also known as forest clearance is defined as the removal of forest or stands of trees from land that is converted to non-forest use in which forest land is converted into farms, ranches or urban may involve converting to use. The most concentrated deforestation occurs in tropical rainforests.

It occurs when humans remove or thin forests for timber or use land where trees stand for crops, grazing, extraction such as mining, oil, or gas, or as populations grow and people move. Development takes place in the form of migration.

Wildlife habitats are fragmented due to deforestation, where native species have to live on remaining habitat islands that are surrounded by disturbed land being used for agriculture and other uses.

Thus, removal of trees and other types of vegetation can reduce the availability of food, shelter and breeding habitat

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¿Qué significa que un gen se exprese?: I. Que se hagan copias de él en forma de ARNm. II, Que se posibilite la síntesis de proteínas. III. Que genere características visibles.

Answers

Answer:

kelfwk   8,,t4

nfdhfku r

Explanation:

what is the rate of person blood in one day​

Answers

Answer:

about 6,000-7,500 liters (1,500-2,000 gallons) of blood daily.

Answer: mark me as a brainlist plz plz plz i really need this

Explanation:

Normal blood in human body

Adults: The average adult weighing 150 to 180 pounds should have about 1.2 to 1.5 gallons of blood in their body. This is about 4,500 to 5,700 mL. Pregnant women: To support their growing babies, pregnant women usually have anywhere from 30 to 50 percent more blood volume than women who are not pregnant

This type of behavior is important to...?

Answers

Maintain homeostasis. Small lizards are cold blooded so they need to sunbathe in the morning to maintain their body temperature so that they can obtain nutrients from digestion.

25 puntos ayudame biologia gracias

Answers

Answer:

no puedo entender tu idioma

reaccionar unos con otros navegar por Internet y buscarlo

carbohydrate are store in animal cell in the form of​

Answers

Answer:

molecule glycogen

Plants store carbohydrates in long polysaccharides chains called starch, while animals store carbohydrates as the molecule glycogen.

Glycogen molecule, which is found in the cytosol of the cell

Nxnznmznznnznznnzndnxnznnxnznxnznznznz

Answers

Hehehdjdjjciifeisodod

Why are NADH and FADH2 necessities in the electron transport chain?(1 point)

They power the electron transport chain.
They power the electron transport chain.

They remove electrons from the electron transport chain.
They remove electrons from the electron transport chain.

They move down the electron transport chain.
They move down the electron transport chain.

They move electrons to the electron transport chain.

Answers

NADH and FADH2 are necessities in the electron transport chain because they power the chain by moving electrons into it.

NADH and FADH2 are both generated at the initial stages of respiration (the Kreb's cycle), a process during which food materials are broken down to generate energy. NADH is generated from NAD+ as a result of the addition of electrons while FADH2 is generated from FAD also due to the addition of electrons.

Once generated, both compounds get transferred into the innermost membrane of the mitochondrion where they travel down the electron transport chain, donating their electrons in the process and creating an electrochemical gradient to generate ATP.

Without NADH and FADH2, there will not be electrons to power the electron transport chain and the generation of ATP would become impossible.

More about the electron transport chain can be found here: https://brainly.com/question/13975046

Answer:

The correct answer is They move electrons to the electron transport chain.

Explanation:

just took the quick check!

hope this helps :)

1. What academic subset of oceanography studies seafloor features and the processes that form them?
O A. Biological oceanography
O B. Marine geology
O C. Physical oceanography
O D. Chemical oceanography

Answers

Biology
Oxnh ma 7 acid

Trees grow more quickly in the ________ than the ________, and the tree rings show up as ________ colored bands.

summer, winter, darker
summer, winter, lighter
winter, summer, lighter
winter, summer, darker

Answers

Answer:

Its summer, winter, darker.

Explanation:

This is the answer I hope it helps you out.

Trees grow more quickly in the summer than in the winter, and the tree rings show up as darker colored bands.

What are seasons?

A season exists the year that stands determined by particular climate conditions. The four seasonsspring, summer, fall, and winter—follow one another regularly. Each includes its light, temperature, and weather designs that repeat yearly.

Summer exists the hottest of the four temperate seasons, happening after spring and before autumn. At or centered on the summer solstice, the earlier sunrise and latest sunset happen, daylight hours exist longest and dark hours exist shortest, with day length falling as the season progresses after the solstice.

Winter, the coldest season of the year, between autumn and spring; the name arrives from an old Germanic word that represents “time of water” and guides to the rain and snow of winter in middle and increased latitudes.

Hence, Trees grow more quickly in the summer than in the winter, and the tree rings show up as darker colored bands.

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which structures in plant cells build protein molecules under the control of the nucleus?
a) cell walls
b) chloroplasts
c)mitochondria
d) ribosomes

Answers

The cell walls, chloroplasts, and mitochondria play important roles in plant cells, they are not directly involved in building protein molecules under the control of the nucleus. D

Ribosomes are the structures in plant cells that build protein molecules under the control of the nucleus.

Ribosomes are tiny, complex molecular machines composed of RNA and protein molecules.

They are involved in the process of protein synthesis, where they facilitate the translation of genetic information from the DNA in the nucleus into functional proteins.

The nucleus of a cell contains the DNA, which serves as the blueprint for building proteins.

However, the actual synthesis of proteins occurs in the cytoplasm, outside the nucleus.

Ribosomes are responsible for this task.

Ribosomes can be found both freely floating in the cytoplasm and attached to the endoplasmic reticulum (ER).

The ones attached to the ER are called rough endoplasmic reticulum (rough ER) due to the presence of ribosomes on their surface.

These ribosomes associated with the rough ER are involved in the synthesis of proteins that are destined for secretion or for integration into the cell membrane.

The process of protein synthesis involves the decoding of the genetic information stored in messenger RNA (mRNA) by ribosomes.

Ribosomes read the mRNA sequence and assemble amino acids into a growing polypeptide chain, forming a functional protein.

Cell walls provide structural support, chloroplasts are responsible for photosynthesis, and mitochondria are involved in cellular respiration. However, the primary site for protein synthesis in plant cells is the ribosomes.

Therefore, the ribosomes (option d) are the structures in plant cells that build protein molecules under the control of the nucleus.

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Which of the following refers to the movement of biochemical and other atomic or molecular substances across biological membranes?
Membrane transport
ATP breakdown
Diffusion
Osmosis

Answers

Answer:

diffusion / osmosis passive transport does not require an input of chemical energy.

Explanation:

What is life process?
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Answers

Answer:

the basic functions performed by living organisms for their survival and body maintenence are called life process.

Explanation:

life process require energy which is provided by nutrition .

basic life processes are : nutrition , respiration , transportation , excretion .

where are genes located in a prokaryotes cell?

Answers

Vas happenin!
Hope your day is good well or night

In the nucleus

Hope this helps *smiles*

Isabel's first car had a manual stick shift. After many years of driving an automatic car, she needs to use her sister's car, which also has a stick
shift. Isabel takes a few minutes to get used to shifting gears. Which memory system Is she using to recall this information?
• A. episodic memory
O B. procedural memory
• C.
semantic memory
)D.
sensory memory

Answers

Answer:

Procedural memory

Explanation:

Isabel has to recall the information about using a manual stick shift. For this reason, she is using long-term, explicit, declarative and episodic memory system.

Human memory system can be broadly classified as: sensory memory, short-term memory and long-term memory.

Long-term memory is used to recall information. If the information are about facts, events and experiences then explicit, declarative and episodic memory helps to recall all information. This will help Isabel to recall all information about driving a car with a manual stick shift and she will be able to drive her sister's car.

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the periodic table houses nobel gas elements in group 18. These elements are highly stable and rarely react with any other elements. What is one possible reason for the stability of the noble gas elements?​

Answers

Answer:

BECAUSE THEY DO NOT HAVE CHARGE

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