Answer: The DNA fragments are separated in an electrophoresis gel and compared with a weight marker, a reference standard containing DNA fragments of known lengths
Explanation:
Gel electrophoresis is a lab technique used to separate DNA according to their size. However, the DNA molecules in the cells are too large to separate through a normal electrophoresis gel, but they can be analyzed if they have previously been fragmented, for example, using restriction enzymes.
Agarose gels (concentration between 0.3% and 2%) are usually used to separate DNA, because they are more porous than polyacrylamide gels.
First, the gel is placed in a chamber with a buffer that allows the conduction of an electric current. One end of that chamber is connected to a negative electrode, while the other end is connected to a positive electrode. So, DNA samples are loaded into a slot next to the negative electrode and an electric current is applied that makes them move through the gel. And, one well is used for a reference standard which has DNA fragments of known lengths. Commercial DNA markers cover different size ranges, so it is important to choose one with good "coverage" in the size range in which we expect to find our fragments. Since the DNA fragments have a negative charge, they will move towards the positive electrode. Thereby, small fragments move through the gel faster than large ones.
At the end, longer fragments will stay close to the negative end as being larger, they move more slowly. And shorter fragments will be closer to the positive end of the gel, because they will move faster.
The next step is to stain the gel with a pigment that binds to the DNA, and the fragments can be seen as bands under UV light allowing us to see the DNA present at different locations along the gel. It should be noted that a single DNA fragment would not be visible. So actually. each band contains a great number of DNA fragments of the same size at the same position.
By comparing a band in a sample with the molecular weight marker, we can determine its approximate size. However, in order to be more precise, we can draw a calibration curve. You can measure the advanced of the electrophoresis front versus the logarithm of the size (lbase pairs) for each band and calculate a regression line. So the advance distances of the samples are interpolated, which will allow you to to calculate the of a specific fragment.
What is the equation for the line of reflection?
Ay
4
B
B
A
A'
2
D
с
с
D'
х
2
4.
6
6
8
10
27
Answer:
the line is perpendicular I think it a good thing that is your choice of a line has to the line y-4 you can make 60-6 with the given point
A red car and a blue car are moving down the road. The two cars are just alike other than the color. Which car has the most kinetic energy?
The one that is moving downhill
The one that is moving faster
The one that is moving uphill
The one that is moving slower
Answer:
the one moving faster
Explanation:
HER1 signaling is triggered by individual HER1 monomers each binding to its ligand followed by homodimerization and autophosphorylation. How do HER2/HER3 heterodimers initiate signaling?
A. Neither HER2 nor HER3 bind ligands, but can form heterodimers that activate HER2 tyrosine kinase which creates a GRB2 binding site.
B. Neither HER2 nor HER3 bind ligands, but can form heterodimers that activate HER2 tyrosine kinase which creates a GRB2 binding site.
C. HER2 and HER3 monomers each bind to its ligand followed by heterodimerization and activation of both HER2 and HER3 tyrosine kinases which create a GRB2 binding site.
D. HER2, but not HER3 binds to its ligand, causing it heterodimerize with HER3. This activates HER3 tyrosine kinase which creates a GRB2 binding site.
E. HER3, but not HER2 binds to its ligand, causing it heterodimerize with HER2. This activates HER2 tyrosine kinase which creates a GRB2 binding site.
Answer:
E. HER3, but not HER2 binds to its ligand, causing it heterodimerize with HER2. This activates HER2 tyrosine kinase which creates a GRB2 binding site.
Explanation:
The receptor tyrosine-protein kinase, also known as human epidermal growth factor receptor 3 (HER3), is a transmembrane tyrosine kinase receptor that doesn't have a kinase domain. HER3 binds to the HER2 protein (another receptor tyrosine kinase receptor) to form a heterodimeric complex. Conversely to the HER3 protein, HER2 lacks a ligand-binding domain. The HER2/HER3 complex triggers the activation of the HER2 kinase domain which then autophosphorylates and thus generates a binding site for the Growth factor receptor-bound protein 2 (Grb2).
2 3x 67 4 y ^6 29 = 7 y ^2 8720 [t + X]
Mrs. Rosales performed a demonstration of a chemical reaction for her science students. In a well-ventilated room, she added a sulfuric acid solution to a beaker containing granulated sugar. The mixture immediately became very hot and released water vapor into the air. The sugar appeared to burn and was transformed into a rapidly growing column of solid black material. The experiment also produced a foul-smelling odor. Which observation about the demonstration does not provide evidence of a chemical reaction? A. The mixture immediately became very hot B.Water vapor was released into the air. C.The mixture released a foul-smelling odor. D.The sugar was transformed into a solid black material
Answer:
D
Explanation:
The observation about the demonstration that does not provide evidence of a chemical reaction is the liberation of water vapor into the air. Thus, the correct option for this question is C.
What is a Chemical reaction?A chemical reaction may be defined as a process that significantly involves the rearrangement of the molecular or ionic structure of a substance, as distinct from an alteration in physical form or a nuclear reaction.
According to the context of this question, the appearance of the mixture that immediately became very hot, the transformation of sugar into a solid black material, and the liberation of a foul-smelling odor are easily estimated or observed during the course of the experiment. But the liberation of water vapor into the air is not visualized with unaided eyes.
Therefore, the observation about the demonstration that does not provide evidence of a chemical reaction is the liberation of water vapor into the air. Thus, the correct option for this question is C.
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How does nondisjunction affect the gametes’ chromosome numbers?
Answer: Nondisjunction can occur during either meiosis I or II, with different results (Figure 7.8). ... If sister chromatids fail to separate during meiosis II, the result is one gamete that lacks that chromosome, two normal gametes with one copy of the chromosome, and one gamete with two copies of the chromosome.
4. Which of the following is not a metamorphic rock?
schist
marble
limestone
anthracite
slate
What does the name “Anthropocene” imply?
Answer:
Human impact on the environment or anthropogenic impact on the environment includes changes to biophysical environments[1] and ecosystems, biodiversity, and natural resources[2][3] caused directly or indirectly by humans, including global warming,[1][4] environmental degradation[1] (such as ocean acidification[1][5]), mass extinction and biodiversity loss,[6][7][8][9] ecological crisis, and ecological collapse. Modifying the environment to fit the needs of society is causing severe effects, which become worse as the problem of human overpopulation continues.[10][11] Some human activities that cause damage (either directly or indirectly) to the environment on a global scale include population growth,[12][13] overconsumption, overexploitation, pollution, and deforestation, to name but a few. Some of the problems, including global warming and biodiversity loss pose an existential risk to the human race, and human overpopulation causes those problems.
Explanation:
The term anthropogenic designates an effect or object resulting from human activity. The term was first used in the technical sense by Russian geologist Alexey Pavlov, and it was first used in English by British ecologist Arthur Tansley in reference to human influences on climax plant communities.[19] The atmospheric scientist Paul Crutzen introduced the term "Anthropocene" in the mid-1970s.[20] The term is sometimes used in the context of pollution emissions that are produced from human activity since the start of the Agricultural Revolution but also applies broadly to all major human impacts on the environment.[21] Many of the actions taken by humans that contribute to a heated environment stem from the burning of fossil fuel from a variety of sources, such as: electricity, cars, planes, space heating, manufacturing, or the destruction of forests.
Hope that Helps:)))))))))))
Which organism lives in a colony with many others of the same cell type?
Answer: The alga Volvox , or multicellular, as in the phylum Bryozoa. The former type may have been the first step toward multicellular organisms.
Explanation:
If the sun were to burn out, what would be the first biological process to
stop?
* Cellular respiration
* photosynthesis
*kreb’s cycle
* Calvin cycle
Chose one of the *
Answer:
Photosynthesis
Explanation:
Photosynthesis uses sunlight directly. Light energy from the sun is used to power the reactions of photosynthesis. Carbon dioxide and water are converted into glucose and oxygen.
This is essential for providing plants with food. They break down this glucose to release cellular energy to power all cellular processes.
The Krebs cycle is a stage of cellular respiration
The Calvin cycle is part of the dark reactions of photosynthesis that do not require sunlight.
Why does the last line create a satisfying ending?
O It makes the reader wonder what happened.
O It describes the writer's frustration with the task.
O It indicates that the writer is well liked.
O It shows that the writer succeeded in his task
Answer:
The answer is D. the person in the comments got it correct :)
Explanation:
i just did it
What are the symptoms and incubation period of an infection by the microbial agent?
Answer:
Explanation: A person with a bacterial infection will often experience redness, heat, swelling, fever, and pain at the site of infection, as well as swollen lymph glands. A rash can indicate a fungal infection of the skin. However, viruses and bacteria may also cause skin conditions and rashes. While the incubation period is 2-10 days, average 3-4 days or sometimes a week.
True or false, all solids are hard
True or false, words used to describe solids are called properties
True or false, liquids take the shape of the container they are in
True or false, liquids are suck down by temperature to the bottom of the container
True or false, when you move a liquid to another container the volume changes
True or false, the temperature at which a solid turns into a liquid is the freezing point
True or false, surgical spirits and ice are solid at room temperature
True or false, the melting point of water is 0°C
True or false, a solid keeps its shape, well liquids have no fix shape or volume
True or false, a solid keeps its shape, well liquids have no fixed shape or volume
True or false, gases Will spread out to fill a container or just keep spreading around
True or false, gases have weight because they are affected by gravity
True or false, in a solid the particles are packed tightly together and vibrate quickly
True or false, as a solid heats up, particles move more quickly and take up more space
True or false, as a solid is heated up eventually eventually cannot keep it shape and becomes a gas
True or false, dissolving solids and liquids creates a solution
True or false, dissolving is when the solid particles disappear from sight
True or false, if it’s all it is dissolved, it can be retrieved by freezing liquid
True or false, when you combine a solid and a liquid dissolving always takes place
True or false, water boils it 100°C ( 212°F ) with bubbles of air
True or false, you need to give water heat (thermal) Energy to turn it into a gas
True or false, changing the amount of heat energy does not change how fast water changes states
True or false, the Gaseous state of water is called water vapor
True or false, lighting a match is a reversible change
True or false, changing boiling water to ice cubes can be reversed
True or false, cooking an egg is a nonreversible change
Answer:
for example when you have an ice it is soild
Are harbor seals a K-selective or R-selective species?
Answer:
They are K-selective
Explanation:
Because harbor seals are mostly captured by humans and are very endangered which are most likely to not survive for the long-term.
What do scientists use to measure the magnitude (strength) of earthquakes?
A.) seismograph
B.) people observations
D.) bathroom scales
Answer:
Option A
Explanation:
The answer is "seismograph" or option A. A seismograph is a tool that records duration and force of a Earthquake when it strikes. It works by responding to ground motions because it's very sensitive to vibrations. So when the device picks up a huge vibration is quickly records how long it lasts and how strong the vibration was.
Hope this helps.
Question 2
Which property of water is the result of an uneven distribution of electrons?
Answer:
Polarity
Explanation:
Water, is a chemical compound with the formula H2O. It posseses properties that makes it a unique substance on Earth. Among these properties is its POLAR NATURE. Polarity of a molecule refers to the separation of electric charges into dipole moment having an opposite charges at the end.
The polarity of water molecule is attributed to the uneven distribution of electrons in the atoms that forms it. Oxygen is a more electronegative atom than hydrogen, hence, it pulls most of the electrons towards itself, making hydrogen atom more electropositive. This forms the polar property of water.
label the parts of the diagram. There is no "E"
1.5-carbon sugar 2.adenine 3.chromosome 4.cytosine 5.phosphate group 6.chromatin
Answer:
Explanation:
A: Cytosine
B. Adenine
C.Phosphate Group
D. Carbon Sugar
F. Chromatin
G. Chromosome
The part of the diagram has been labelled correctly here:
A: Cytosine
B. Adenine
C.Phosphate Group
D. Carbon Sugar
F. Chromatin
G. Chromosome
What is nucleotide?It is the sub units and buIlding blocks of DNA. It is made up of a five-sided sugar, phosphate group and then a nitrogen base.
These groups make the backbone of the DNA helix. If you look at a DNA helix, they make the side of the ladder or the side portion. They connect to a nitrogen base which make the steps of the ladder. The type of sugar that is used in a DNA helix is called deoxyribose.
Nitrogen bases are the molecules that make up the steps of the ladders. There are four different nitrogen bases, namely; Guanine, Thymine,Adenine and Cytosine.
Pyrimidines are compounds that make a single 6-sided ring. Examples of pyrimidines are Cytosine and Thymine. Purines on the other hand make 5-sided and 6-sided rings.
Therefore, The part of the diagram has been labelled correctly here:
A: Cytosine
B. Adenine
C.Phosphate Group
D. Carbon Sugar
F. Chromatin
G. Chromosome
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When you quickly shine the light into the right eye and the pupil constricts why also does the left pupil constrict?
Answer: When the left eye is stimulated by light, the right pupil constricts, because the afferent limb on the left and the efferent limb on the right are both intact. The right direct reflex is intact. When light is shone into right eye, right pupil constricts.
Explanation:
what will be the consequence if humans continue to over-hunt elephants for their ivory
Answer:
Extinction
Explanation:
Answer:
extinction if they are hunted so much
What is the density? Mass = 44g ... Volume = 44L
mass = 44g
volume= 44L
1 liter = 1000cm^3
hence 44L = 44000cm^3.
Density = mass/volume
= 44/44000
= 0.001g/cm^3.
Who tried to help Canadian rebellions?
Americans
Intuits
French
British
Answer: Americans
Explanation: With the help of American volunteers, a second rebellion was launched in November 1838. However, it too was poorly organized and was quickly put down. It was followed by further looting and devastation in the countryside. The two uprisings left 325 people dead, all of them rebels except for 27 British soldiers. Nearly 100 rebels were captured. After the second uprising failed, Papineau left the US for exile in Paris.
Which of the following is a cost of mining aluminum from new bauxite
deposits?
A. It requires a large amount of energy.
B. It can be recycled over and over again.
C. It takes less energy to transport this lightweight metal.
D. It helps preserve plants and animals in rain forests.
Answer:
The process is high-energy intensive (the alternative answer)
Explanation:
What is the answer and give me an explanation??!?!?!
Answer:
beryllium (Be). Beryllium metal is relatively unreactive at room temperature, particularly in its massive form.
Explanation:
Answer all of them please ty
Answer:
23) 2 (egg)
24) 4 (fertalization)
25) 3 (8)
Explanation:
56:52 Which process is characterized by the movement of particles from an area of high concentration to an area of low concentration across the plasma membrane without the use of energy? hypertonic transport active transport passive transport dynamic equilibrium
Answer:
The movement of particles from a high concentration area to a low concentration area through the plasma membrane without requiring energy is done by the process of passive transport.
Explanation:
Passive transport is a transmembrane transport mechanism that does not require energy and depends on a gradient of particle concentration.
The plasma membrane is a semi-permeable barrier to the passage of substances, which require transport through them by various transport mechanisms, including passive transport.
The different concentrations of particles on each side of the membrane create a gradient, which makes the movement of particles from the area of highest concentration to the area of lowest concentration, which in some circumstances is a passive transport mechanism, such as simple diffusion or facilitated diffusion.
The other options are not correct because:
Hypertonic refers to a medium where there is a high concentration of particles. Active transport requires energy. Dynamic equilibrium refers to the continuous exchange of substances that occurs between the interior and exterior of the cells.C. Passive Transport.
The person above was correct, that is where I got this answer. If you would like an explanation read theirs.
A sailboat travels 35 km south in 5 hours. Which of the following statements describes the motion of the sallboat?
Answer:
7km/hr
Explanation:
What determines climate patterns?
Answer:
the answer is below
Explanation:
The two most important factors in the climate of an area are temperature and precipitation. The yearly average temperature of the area is obviously important, but the yearly range in temperature is also important. ... Some areas have about the same rainfall throughout the year.
How many tissue layers does an embryo have?
Answer:
they have 3 layers
Explanation:
endoderm, the ectoderm, and the mesoderm.
Genes that are linked together ______.
Answer:
combine to form like traits
Explanation:
the genes are made from DNA which is made from chromosomes which hold the dominant and recessive traits of and offspring(s) (if this was incorrect, I apologize)
All of the following are examples of limiting factors except
A) Food
B) Soil
C) Space
D) Weather conditions
Answer:
soil is the answer
7. The periodic table has changed over time as new elements have been added to it. Which
is the most likely reason scientists update the periodic table?
A. Scientists rename old elements.
B. Scientists discover new elements.
C. Stars produce new elements that travel to Earth.
D. Past mistakes are corrected in identifying elements.
Answer:
it will be the answer B. scientists discover new elenents.
The most likely reason that scientists update the periodic table is that Scientists discover new elements.
What is periodic table?All identified chemical elements are arranged in rows (referred to as periods) and columns (referred to as groups) in the periodic table of chemical elements, also known as the periodic table, in ascending order of atomic number.
The periodic table is used by scientists to quickly refer to details about an element, such as its atomic mass and chemical symbol. Scientists can identify trends in element properties like electronegativity, ionization energy, and atomic radius thanks to the periodic table's arrangement.
Many scientists worked on the issue of grouping the elements, but Dmitri Mendeleev is generally recognized as the periodic table's creator. In 1869, he produced the first version of the periodic table.
Therefore, The most likely reason that scientists update the periodic table is that Scientists discover new elements.
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