15. Assuming the fluorescence threshold is set to be 1. Which gene has the highest gene expression rate? What is the Ct value? Gene with highest expression = ______________ Ct value = _______________

Answers

Answer 1

Answer:

The correct answer is - Gene 1 and 14.5 (or 14).

Explanation:

The cycle threshold is denoted as Ct value. It is the cross point at the threshold line meets with fluorescence signal reaction curve. In the given RTpcr experiment, the threshold line is fluorescence intensity 1 thus, Ct value for each gene will be the point at which each curve meets the fluorescence intensity line at 1. Ct value of is inversely proportional to amount of nucleic acid in the given sample.

Here, the Ct for gene 1 is the lowest, so the amount of DNA would be highest.

Gene with highest expression= Gene 1

Ct value= 14.5

15. Assuming The Fluorescence Threshold Is Set To Be 1. Which Gene Has The Highest Gene Expression Rate?

Related Questions

Importance of antimicrobial emulsion​

Answers

Answer:

Citral is a typical essential oil used in the food, cosmetic, and drug industries and has shown antimicrobial activity against microorganisms. Citral is unstable and hydrophobic under normal storage conditions, so it can easily lose its bactericide activity. Nanoemulsion technology is an excellent way to hydrophilize, microencapsulate, and protect this compound. In our studies, we used a mixed surfactant to form citral-in-water nanoemulsions, and attempted to optimize the formula for preparing nanoemulsions. Citral-in-water nanoemulsions formed at So 0.4 to 0.6 and ultrasonic power of 18 W for 120 seconds resulted in a droplet size of < 100 nm for nanoemulsions. The observed antimicrobial activities were significantly affected by the formulation of the nanoemulsions. The observed relationship between the formulation and activity can lead to the rational design of nanoemulsion-based delivery systems for essential oils, based on the desired function of antimicrobials in the food, cosmetics, and agrochemical industries.

Transcription and Translation Questions Note: Answer the following questions without taking start codon into consideration. 6. The template DNA sequence is 5’ ACCTGAGTC 3’, what is the transcribed mRNA sequence from 5’ to 3’?

Answers

3’ UGGACHCAG 5’ is the answer.


How does disease and parasitism cause extinction?

Answers

Explanation:

Invading infectious diseases can, in theory, lead to the extinction of host populations, particularly if reservoir species are present or if disease transmission is frequency-dependent.

Answer:

Abstract. Infectious disease, particularly severe infectious disease, is widely recognized as a cause of biological population fluctuation or decrease. However, it is not usually thought to be a major factor in triggering species extinction or endangerment.

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A genetically heterogeneous population of rice has a mean in the number of days to maturation of 30. Selection for decreased period of maturation is carried out for one generation. The average period to maturation among the plants selected as parents for the next generation is 25 days. F1 plants mature on average in 27 days. Estimate the narrow sense heritability.

Answers

Answer:

h² = 0.6

Explanation:

Before answering the question, we need to know a few concepts.

Artificial selection is the selecting practice of a specific group of organisms in a population -that carry the traits of interest- to be the parents of the following generations.

Parental individuals carrying phenotypic values of interest are selected from the whole population. These parents interbreed, and a new generation is produced.

The selection differential, SD, is the difference between the mean value of the trait in the population (X₀) and the mean value of the parents, (Xs). So,  

SD = X₀ - Xs

Heritability in the strict sense, h², is the genetic component measure to which additive genetic variance contributes. The heritability might be used to determine how the population will respond to the selection done, R.  

h² = R/SD

The response to selection (R) refers to the metric value gained from the cross between the selected parents. R can be calculated by multiplying the heritability h², with the selection differential, SD.  

R = h²SD  

R also equals the difference between the new generation phenotypic value (X₁) and the original population phenotypic value (X₀),  

R = X₀ - X₁

-------------------------------------------------------------------------------------------------------------

Now that we know these concepts and how to calculate them, we can solve the proposed problem.

Available data:  

You are selecting rice´s decreased period of maturation. The population of rice has a mean maturation time of 30 days → X₀ Parental selected average period to maturation is 25 days → Xs F1 plants mature on average in 27 days → X₁ N arrow sense Heritability → h²

According to what we sow previously, we need to find out the value of h².

We know that h² = R/SD, so we need to get R and SD first.  

R = X₁ - X₀

R = 27 - 30

R = -3

SD = Xs -  X₀  

SD = 25 - 30

SD = -5

Knowing this, we can calculate h²

h² = R/SD

h² = -3/-5  

h² = 0.6

 

diagram of a tick with labels​

Answers

Answer:

I posted some pictures, I hope it helps!

The structure in the male reproductive system of rabbit which releases nutritious secretions for sperms
is called
O a.
Seminal vesicles
O b. Spermatogonia vesicles
Ос.
Cowper's vesicles

Answers

Answer:

I think the answer is A

Explanation:

A search of the definitions of each option

2. What kind of plants contain tubular structures that are used to conduct water from the soil through the roots?
non-vascular plants
seedless plants
seed plants
vascular plants

Answers

Answer:

Vascular plants

Explanation:

I took the quiz

is mucous an enzyme or harmone​

Answers

Answer:

It's an Enzyme.

Explanation:

It is a viscous colloid containing inorganic salts, antimicrobial enzymes (such as lysozymes), immunoglobulins (especially IgA), and glycoproteins such as lactoferrin and mucins, which are produced by goblet cells in the mucous membranes and submucosal glands.

How do make a sperm cell go in to a woman

Answers

During sex, sperm cells travel through the vagina to the uterus and fallopian tubes. In the fallopian tube, the sperm meets the egg that was released from the ovary during ovulation.

If a physicist performs an experiment, who would likely try to replicate it?

The physicist himself
Other physicists
The family of the physicist
No one, because an experiment can never be replicated

Answers

Answer:

The physicist himself

Explanation:

Replication in an experiment means to repeat an experiment as many times as possible using the same conditions. This is done to minimize error and ascertain the reliability of the experimental outcome.

An experimenter is responsible for repeating his/her experiments. According to this question, a physicist performs an experiment. The physicist himself should replicate his experiment.

Marianela takes a huge drink of her coffee, assuming that it is at a tolerable temperature, and the heat sears her mouth. Although the pain is so great that her reflex is to spit out the coffee, she does not do so. The reflex to spit out the coffee is inhibited at the

Answers

Answer:

This question lacks options, options are:

A) glial cell

B) interneuron

C) sensory neuron

D) motor neuron.  

The correct answer is D.

Explanation:

The brain sends instructions to the muscles that control voluntary movement through nerve cells called motor neurons. The function of the motor neuron is basically to carry the information that comes from the descending pathways of the spinal cord, in order to generate an activation in the tissues of the skeletal and visceral muscles of all parts of the body. Motor neurons need the information sent to them by sensory or efferent neurons. Since in order to perform the appropriate muscle movements for the situation, our brain must receive information from the outside. By sending sensory stimulation through interoreceptors, motor neurons can regulate visceral activity, stimulating or inhibiting the activity of their effector tissues. In this way, our nervous system integrates the information from both types of neurons and allows us to move and react according to the demands and circumstances of our external context.

Which is our body part help us to maintain balance.

Answers

Answer:

cerebellum

Explanation:

The cerebellum is a small part of the brain positioned at the back of the head, where it meets the spine, which acts as the body's movement and balance control centre.

please mark me as brainliest

Tai không chỉ có vai trò để lắng nghe mà còn hỗ trợ trong việc giữ cân bằng

Which of the following would provide the best evidence that species A and species B have a common ancestor?
O A. The limbs of species A perform a function that is similar to the limbs of species B, but they have a different structure.
OB. Species A has limbs, and species B does not have limbs.
O c.
The limbs of species A and species B have a different structure and function.
OD
The limbs of species A have a similar structure to the limbs of species B, but they perform a different function.

Answers

Answer:

I believe the answer is D

Explanation:

Answer:

B

Explanation:

The answer is B on Plato. Just took the test and got it right.

Which BEST
describes the
amount of air in
a typical soil?
A. about 90%
B. hardly any
C. about 25%

Answers

Answer:

C

Explanation:

it's about 20%-30%, so C would appropriately about that range since it the best answer :)

hope it helps

What bug is this please help I found it on the ground and want to make it my pet I need answers ASAP what bug is this please

Answers

Maybe European earwig or normal earwig kinda hard to see

sexual reproduction study's

Answers

Explanation:

join

Goole meet

rej-evxq-xcb

Anaerobic Respiration of Yeast
1. Describe at least one part of the experiment procedure you thought was
essential for getting good results. Did you find that certain steps in the
procedure had to be followed carefully to get consistent results? If you wanted
better results, do you think there is a step that could have been added to the
procedure?
2. Discuss your thoughts on the overall lab design. Did it help you understand the
concepts better, or did it raise more questions? Do you think you could have
designed a better experiment? If so, explain how and then discuss it with your
classmates. Share some of your knowledge with them to learn a little more
about this experiment.

Answers

Anaerobic respiration of yeast produces ethanol and carbon dioxide, which can be experimentally tested by determining the rate of CO2 production in a closed system.

What is anaerobic respiration?

Anaerobic respiration is a series of chemical reactions by which certain organisms can produce energy (ATP) in absence of oxygen.

Anaerobic respiration is useful in experimental settings to determine the survival rate of yeast, which can be determined by measuring the concentration of products of this process.

The experimental procedure requires the use of a closed system to determine the rate of change between reactants (glucose) and products (ethanol + carbon dioxide).

In conclusion, Anaerobic respiration can be experimentally tested by determining the rate of CO2 production.

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ANOVA is a technique that permits the comparison of the equality of means for multiple populations. Thus, 3 means could be compared and a determination of whether or not one of the means is significantly different from others can be made. This technique is extremely useful, but, as is always the case, the application requires a firm understanding of models assumptions. Consider the following scenario. A meticulously determined random sample of 200 observations is taken from a very large population. This data divides into 4 groups or factor levels, and a numerical measure for each observation is recorded. The population distribution of the measure appears to be slightly skewed positive. The 4 groups have the following number of observations 30, 100, 15 and 55. Which of the following statements leads to the greatest concern in using ANOVA as an analytical technique.
a. Unequal sample sizes among groups can seriously affect the power of the test.
b. Slightly skewed positive distribution of population measures seriously affect the power of the test.
c. In this scenario, I should be concerned about whether the data has been gathered in a biased fashion.
d. Four is not a sufficient number of groups into which to divide the data.

Answers

Answer:

c. In this scenario, I should be concerned about whether the data has been gathered in a biased fashion.

Explanation:

ANOVA is a statistical method which separates observed variance data into different components to use. One way ANOVA is used for three or more groups of data. The greatest concern in ANOVA is that the data gathered should not be biased.

Why does each trophic level have a smaller amount of organisms or biomass than the one below it? A. Because higher trophic levels have lower survival rates for offspring. B. Because each trophic level has larger organisms than the one below it. C. Because the higher trophic levels have to hunt the lower trophic levels, so they- use most of the energy that they gain from eating. D. Because each trophic level is only absorbing 10% of the previous, so there is not enough energy to sustain larger numbers.

Answers

Each trophic level has a smaller amount of organisms or biomass than the one below it because it is only absorbing 10% of the energy from the lower level, so there is not enough energy to sustain larger numbers.

A trophic level refers to a level in the food chain consisting of the same or biologically similar organisms. Examples of trophic levels include producer, consumer, and decomposer.Organisms in the lower trophic level serve as food for organisms in the next trophic level of the food chain.Only about 10% of the energy derivable from one trophic level gets to the next trophic level, the remaining is lost as heat during metabolic processes.Hence, the number of organisms progressively decreases as we climb higher in the food chain. The limit to the amount of energy transferred as we move higher means that the number of individuals that can be sustained will also be limited.

The correct option would, therefore, be D.

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define structure and function

Answers

Answer:

(biology) An arrangement or organization of parts to form an organ, system, or living thing. (2) (ecology) A network or a hierarchy of interrelated parts of a system. ... (4) The formation or mode of construction of a body or system from distinct units.

A couple owned a fine pure-bread albino female poodle (an autosomal recessive phenotype) and wanted to mate her to produce a litter of white puppies. They took her to a breeder, who said he would mate the female with an albino stud male, also from a pure stock. When six puppies were born, they were all black, so the couple sued the breeder, claiming that he had replaced the stud male with a black dog, producing six unwanted puppies. You are called as an expert witness, and the defense asks you whether it is possible to produce black offspring from two pure-breeding recessive albino parents. What testimony do you give

Answers

Answer:

No, it is not possible to produce black offspring from two pure-breeding recessive albino parents. If he would have mated the female with a white male, the whole progeny should be white.

Explanation:

Due to technical problems, you will find the complete explanation in the attached files.

Which of the following changes to Earth is caused predominantly by living organisms?
A. The production of dirt.
B. The release of free nitrogen into the atmosphere.
C. The release of sulfur dioxide into the atmosphere.
D. The movement of air currents by convection cells.

Answers

Which of the following changes to Earth is caused predominantly by living organisms?

A. The production of dirt.B. The release of free nitrogen into the atmosphere.C. The release of sulfur dioxide into the atmosphere.D. The movement of air currents by convection cells.

The production of dirt is cause predominantly by living organisms

This production can be caused through emission of pollution in the form of air, water, soil, noise or e-waste.

What is Pollution?

Pollution is defined as the introduction of unwanted harmful elements in the form of release of gaseous toxic substances which result into polluting the environment.

These pollutants can be both natural and anthropogenic.

Natural pollutants are volcanic ash, river sediments

Anthropogenic pollutants are chemicals released from industries vehicular emission.

Majorly pollution are of four types- Air, Water, Soil and Noise. Recently another pollution in the form of e-waste is also prominent.

Effects of pollution on environment:

1. Biomagnification describes situations where toxins (such as heavy metals)  passes through trophic levels, becoming exponentially more concentrated in the process.

2. Carbon dioxide emissions cause ocean acidification, the ongoing decrease in the pH of the Earth's oceans as CO2 becomes dissolved.

3. The emission of greenhouse gases leads to global warming which affects ecosystems in many ways.

4. Nitrogen oxides are removed from the air by rain and affect the land which can change the species composition of ecosystems.

5. Smog and haze can reduce the amount of sunlight received by plants to carry out photosynthesis and leads to the production of tropospheric ozone which damages plants.

6. Sulfur dioxide and nitrogen oxides can cause acid rain which lowers the pH value of soil.

7. Organic pollution of watercourses can deplete oxygen levels and reduce species diversity.

Rest three options are majorly natural process.

Option B focuses on release of free nitrogen into atmosphere which is one of the important step of Nitrogen Cycle.

Option C focuses towards release of Sulfur Dioxide into atmosphere. This process is caused either naturally or anthropogenically. Thus cant blame solely the anthropogenic factor for its release.

Option C is the total natural process i.e. Climatological Process where air currents are regulated by Convection Cells and regulate based on different natural factors.

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what is the function of folds and villi

Answers

Answer:

The inner wall of the small intestine is covered by numerous folds of mucous membrane called plicae circulares. The surface of these folds contains tiny projections called villi and microvilli, which further increase the total area for absorption.

Explanation:

Hope this helps

Which statement best describes a line graph?
A. It shows a line passing through scattered data points.
B. It shows a line that connects a series of data points.
C. It shows data as proportions of a whole.
D. It shows the locations of errors in data.

Answers

Answer:

B

Explanation:

I just did it

A line graph shows a line that connects a series of data points

what is line graph ?

A line graph is a unique type of graph which is used in statistical application which represents the change in a quantity with respect to another quantity.

The price of different flavors of chocolates varies which can be represented, these variation is plotted in a two-dimensional XY plane.

If the relation include two measures can be presented by using a straight line in a graph called as linear graphs or a linear graph.

There are different types of the line graph, Simple Line Graph refers to Only one line is plotted on the graph, Multiple Line Graph where More than one line plotted on the same set of axes.

Compound Line Graph refers to the information can be  divided into two or more types of data and this line graph is called a compound line graph which can be drawn to show the component.

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Considering your knowledge of carbohydrates, evaluate the use of chitin as a component of health foods.

Answers

Answer:

Carbohydrates may be defined as energy-rich foods such as sugars and starches. if consumed in excess or not properly metabolized in the body, carbohydrates may lead to obesity and which may also lead a person to severe number of diseases.

Chitins are components of the exoskeletons of foods such as shrimps, crabs and snails, and insects.

Chitin's use as a component in healthy foods is based on its health benefits.

For example it promotes weight loss , prevents obesity, relieving constipation and preventing inflammation associated with refined carbohydrates, cookies and candies

Which of the following is a characteristic of the plasma membrane?


To protect the cell's internal contents, water is the only substance that can freely pass through the plasma membrane.


Only small molecules are allowed into and out of the cell by the plasma membrane.


Its selective permeability allows certain molecules, such as oxygen and carbon dioxide, to flow freely in and out of the cell while controlling the flow of other molecules.


The structure of the phospholipids forms a stiff protective outer shell around the cell's internal contents.

Answers

Answer:

The plasma membrane forms a barrier between the cytoplasm and the environment outside the cell. A main characteristic of the plasma membrane is selective permeability

The following is a characteristic of the plasma membrane: Its selective permeability allows certain molecules, such as oxygen and carbon dioxide, to flow freely in and out of the cell while controlling the flow of other molecules, which is the third option.

What is the importance of the plasma membrane in cells?

The plasma membrane acts as a barrier in the cell, controlling the movement of molecules as well as some gases such as oxygen, carbon dioxide, and water that pass through it but not larger and more polar molecules. The importance of this is that it regulates and controls the inflow and outflow of substances such as hormones, etc.

Hence, the following is a characteristic of the plasma membrane: Its selective permeability allows certain molecules, such as oxygen and carbon dioxide, to flow freely in and out of the cell while controlling the flow of other molecules, which is the third option.

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On the basis of their use and development, Natural resources can be classified as ________ and ________​

Answers

Answer:

renewable , non-renewable

Explanation: natural resources can be classified as either renewable or non-renewable depending on whether or not they replenish naturally.

Which element is necessary to make proteins for plant growth?
FAst

Answers

Answer:

Nitrogen (N), Phosphorus (P), and potassium (k)

what is the difference of biology and human and social biology ???​

Answers

Answer:

Biology is the branch of science.

Human biology is the branch of biology that focuses on human beings and human population.

Social biology is the branch of biology that is the study of social behaviour of animal and human.

Answer:

Biology is the study of living things.

Social biology studies human and animal behaviour.

:):):):):):):):):):):):):):):):):):):):):):):)

Explanation:

state 6 importance of respiration​

Answers

Answer

Respiration is important because it produces energy that is essential for the normal functioning of the body.Respiration provides cells with oxygen and expels toxic carbon dioxide.It provides pure oxygen to alive and for proper functions.It is very important to live alive.It supply and remove oxygen and carbon dioxide. It has a leading role in functioning in the human body.
Other Questions
Country A and Country B have had an informal trading arrangement for many years wherein merchants on the border of the countries may freely trade goods without the restriction of fees. Which of the following is true?a. The government of Country A may not arbitrarily begin to charge fees for items brought in from Country B.b. Country A and Country B have a binding agreement to this arrangement under customary international law.c. Country A and Country B have no binding agreement under customary international law.d. More than one response is correct. Will give BRAINLIEST to the correct answer.Angelica Reardon received a 4-year non-subsidized student loan of $18,000 at an annual interest rate of 6.8%. What are Angelicas monthly loan payments for this loan after she graduates in 4 years? (Round your answer to the nearest cent.) For the following reaction, 4.77 grams of carbon (graphite) are allowed to react with 16.4 grams of oxygen gas. carbon (graphite) (s) + oxygen (g) carbon dioxide (g) 1. What is the maximum amount of carbon dioxide that can be formed?2. What is the FORMULA for the limiting reagent? 3. What mass of the excess reagent remains after the reaction is complete? De que esta hecho el sol? plisss ayuda.no necesito un texto de 100 reglones, puede ser resumido en solo 2 renglones resolve (5x+4) /( (x-4)( x+2)) Use the point-slope form from the previous question and fill-in the following table of values.The point-slope equation went through the following 2 points: (0, -1) and (1, 2)(0, -1)(1, 2)(2, )(3, ) Penelope earned $50 in May. She earned $60 in June.What is the percent increase or decrease in Penelope's monthly earnings?O A. 30% decreaseO B. 25% increaseO C. 20% decreaseO D. 20% increase Which literary work was written for the ourpose of providing information about native American customs and language Which answer shows 0.00897 written in scientific notation?From what I heard it's not D Why should political parties have intra party elections from book I am Malala.why?Radio Mullah puts in so much effort to discourage people from going to school? please please help me please help me Summarize the roles, powers, and operation of the three main branches of government. IN YOUR OWN WORDS PLEASE Thanks and reqestsYou recently stayed in a hotel. You left a coat in the wardrobe in your room. You wrote to the hotel, asking them to send the coat to you, and also asking for a brochure. Write to the hotel again, thanking them for sending the coat and asking for a brochure. The frequency table represents the job status of a number of high school students. A 4-column table with 3 rows titled job status. The first column has no label with entries currently employed, not currently employed, total. The second column is labeled looking for job with entries 12, 38, 50. The third column is labeled not looking for a job with entries 28, 72, 100. The fourth column is labeled total with entries 40, 110, 150. Which shows the conditional relative frequency table by column? A 4-column table with 3 rows titled job status. The first column has no label with entries currently employed, not currently employed, total. The second column is labeled looking for a job with entries 0.3, nearly equal to 0.33, 1.0. The third column is labeled not looking for job with entries 0.7, nearly equal to 0.65, 1.0. the fourth column is labeled total with entries nearly equal to 0.27, nearly equal to 0.73, 1.0. A 4-column table with 3 rows titled job status. The first column is blank with entries currently employed, not currently employed, total. The second column is labeled Looking for a job with entries 0.12, 0.38, 050. The third column is labeled not looking for a job with entries 0.28, 0.72, 1.00. The fourth column is labeled total with entries 0.4, 1.1, 1.5. A 4-column table with 3 rows titled job status. The first column has no label with entries currently employed, not currently employed, total. The second column is labeled looking for a job with entries 0.24, 0.76, 1.0. The third column is labeled not looking for a job with entries 0.28, 0.72, 1.0. The fourth column is labeled total with entries nearly equal to 0.27, nearly equal to 0.73, 1.0. A 4-column table with 3 rows titled job status. The first column has no label with entries currently employed, not currently employed, total. The second column is labeled looking for job with entries 0.08, nearly equal to 0.25, nearly equal to 0.33. The third column is labeled not looking for a job with entries nearly equal to 0.19, 0.48, nearly equal to 0.67. The f What is the percent increase each year for the investment? I need help this question is worth 20 points and cant fail this class 1. The sum of x and 10 is -1. Find x. Read, reflect and writewhat are your favourite things about your home and the area in which you live What are the root(s) of the quadratic equation whose related function is graphed below? Need help with this, don't understand it. we weren't taught how to do this